CD8A Mouse Monoclonal Antibody [Clone ID: CVS8]

CAT#: SM2207R

CD8A mouse monoclonal antibody, clone CVS8, PE

Product Images

Staining of Equine peripheral blood lymphocytes with Mouse anti Horse CD8 antibody PE conjugated Cat.-No SM2207R (Clone CVS8)

Specifications

Product Data

• Clone Name: CVS8
• Applications: FC
• Recommended Dilution: Flow Cytometry: Use 10 µl of neat diluted CD8 antibody to label 10⁶ cells in 100 µl.
• Reactivities: Donkey, Equine
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Equine PBMC.
  Fusion Partners: Spleen cells from immunised BALB/c mice were fused with cells of the X63-Ag8.653 myeloma cell line.
• Specificity: This monoclonal CVS8 antibody recognises the Equine homologue of the Human CD8 cell surface antigen which is expressed by a subset of T lymphocytes.
  A study undertaken using CVS8 to identify CD8 on several wild african equid species indicates that the CVS8 clone recognises Somali wild ass (Equus asinus) but not Grévy's Zebra (E. grevyi) or Hartmann's Mountain Zebra (E. zebra) (Ibrahim, S, 2007).
• Formulation: PBS
  Label: PE
  State: Lyophilized purified IgG fraction from tissue Culture Supernatant
  Stabilizer: 1% BSA, 5% Sucrose
  Preservative: 0.09% Sodium Azide
• Reconstitution Method: Restore with 1.0 ml distilled water
  Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. We recommend that the vial is gently mixed after reconstitution.
• Purification: Affinity Chromatography on Protein A
• Conjugation: PE
• Storage: Prior to and following reconstitution store the antibody at 2-8°C.
  DO NOT FREEZE!
  This product is photosensitive and should be protected from light.
• Stability: Shelf life: one year from despatch.
• Background: The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell to cell interactions within the immune system. The CD8 antigen, acting as a coreceptor, and the T cell receptor on the T lymphocyte recognize antigen displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The functional coreceptor is either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains.
• Synonyms: CD8 alpha chain, CD8A, MAL