LAMP2 Rat Monoclonal Antibody [Clone ID: M3/84]

CAT#: SM2211PS

LAMP2 rat monoclonal antibody, clone M3/84, Purified

Specifications

Product Data

• Clone Name: M3/84
• Applications: FC, IHC, IP
• Recommended Dilution: Flow Cytometry: 1/50 - 1/100; Use 10µl of the suggested working dilution to label 10e6 cells in 100µl; Results may be enhanced using permeabilisation.
  Immunohistochemistry on frozen sections.
  Immunoprecipitation.
• Reactivities: Mouse
• Host: Rat
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Glycoproteins purified from mouse peritoneal macrophage membranes. Spleen cells from immunised Lewis rats were fused with cells of the mouse P3-NSI/1-Ag4-1 myeloma cell line.
• Specificity: This antibody recognises CD107b, also known as MAC-3 and LAMP-2.
• Formulation: PBS, pH7.4 containing 0.09% Sodium Azide
  State: Purified
  State: Liquid purified IgG
• Concentration: lot specific
• Purification: Affinity chromatography on Protein G
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Background: CD107b is a transmembrane glycoprotein that is associated with lysosomal membranes and is primarily expressed on mononuclear phagocytes. Expression of CD107b does vary between cell populations and the molecular weight of CD107b can vary between 92-120kD. Studies suggest that CD107b may be involved in leucocyte adhesion.
• Synonyms: LAMP-2, LAMP-2C, LAMPB