Il2rb Rat Monoclonal Antibody [Clone ID: TM-BETA1]

CAT#: SM2224LE

Il2rb rat monoclonal antibody, clone TM-BETA1, Low Endotoxin

Specifications

Product Data

• Clone Name: TM-BETA1
• Applications: FC, FN, IP
• Recommended Dilution: Flow Cytometry: 1/10 - 1/50; Use 10µl of the suggested working dilution to label 10e6 cells in 100µl.
  Immunoprecipitation.
  Functional Assays.
• Reactivities: Mouse
• Host: Rat
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Cells from murine IL-2RB+ transformant TART-mBeta. Lymph node (cells from immunised Sprague Dawley rats were fused with cells of the mouse PA1 myeloma cell line.
• Specificity: This antibody recognises the CD122 cell surface glycoprotein. Clone TM-beta 1 is reported to block IL-2 high affinity binding to the IL-2R beta receptor. We recommend the use of SM2224LE for this purpose.
• Formulation: PBS, pH7.4
  State: Low Endotoxin
  State: Liquid purified IgG
• Concentration: 1.0 mg/ml
• Purification: Affinity chromatography on Protein G
• Database Link:
  Entrez Gene 16185 Mouse
• Background: CD122 is a 75-85kD molecule also known as interleukin-2 receptor (IL-2R) beta chain. In mouse, CD122 is primarily expressed on natural killer (NK) cells and at lower levels on lymphocyte subsets, monocytes and macrophages. CD122 can associate with the IL-2R alpha chain (CD25) and CD132 to form a functional high affinity receptor complex that binds IL-2. CD132/CD122 heterodimers can also be formed which bind IL-2 with intermediate affinity. IL-2 binding to the IL-2R is important in the generation and differentiation of T lymphocytes and NK cells.
• Synonyms: Interleukin-2 receptor subunit beta, IL-2 receptor subunit beta, IL2 Receptor beta, P70-75