CD45 / LCA Mouse Monoclonal Antibody [Clone ID: UM16-6]

CAT#: SM2229R

CD45 / LCA mouse monoclonal antibody, clone UM16-6, PE

Specifications

Product Data

• Clone Name: UM16-6
• Applications: FC
• Recommended Dilution: Flow Cytometry: Neat - 1/5; Use 10µl of the suggested working dilution to label 10e6 cells in 100µl.
• Reactivities: Chicken
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: COS cells transfected with CD45 gene.
• Specificity: This antibody recognizes CD45 (leucocyte common antigen).
• Formulation: Containing 0.09% Sodium Azide and 1% Bovine Serum Albumin
  Label: PE
  State: Lyophilized purified IgG
  Label: R. Phycoerythrin (RPE)
• Reconstitution Method: Restore with 1.0 ml distilled water
• Concentration: 0.1 mg/ml
• Purification: Affinity chromatography on Protein G
• Conjugation: PE
• Background: CD45 is a heavily-glycosylated transmembrane protein tyrosine phosphatase (PTPase) expressed by all nucleated cells of haematopoietic origin. Variation in the expression of a particular CD45 isoform, is regulated during the haematopoietic development of the different cell lineages. CD45 is essential for antigen-induced signal transduction through the antigen receptor and as with other PTPase family members, acts in balance with protein tyrosine kinases, causing the dephosphorylation of negative regulatory tyrosine sites. Studies have indicated that dephosphorylation by CD45, is required for the activation of the src-family kinases p56lck and p59fyn. Investigations into the properties of CD45 in chicken models are limited, but there is evidence of the existence of an additional cysteine residue near the transmembrane region. The overall domain structure between mammalian and chicken CD45 appears to be conserved, but the sequence homology between the extracellular regions is very low.
• Synonyms: PTPRC, Leukocyte common antigen, L-CA, T200