MADCAM1 Mouse Monoclonal Antibody [Clone ID: 314G8]

CAT#: SM2237PS

MADCAM1 mouse monoclonal antibody, clone 314G8, Purified

Specifications

Product Data

• Clone Name: 314G8
• Applications: ELISA, FC, FN, IHC, WB
• Recommended Dilution: Immunohistochemistry on frozen sections: The typical starting working dilution is 1:50.
  Immunohistochemistry on paraffin sections: The typical starting working dilution is 1:50.
  Flow cytometry: The typical starting working dilution is 1:50.
  Functional assays.
  Immunoassays.
  Western blot: The typical starting working dilution is 1:50.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Specificity:

  The monocolonal antibody 314G8 reacts with human mucosal addressin cell adhesion molecules-1 (MAdCAM-1), a key player in mediating the infiltration of leukocytes into chronically inflamed tissue.

• Formulation: PBS
  State: Purified
  State: Liquid 0.2 µm filtered Ig fraction
  Stabilizer: 0.1% bovine serum albumin
• Concentration: 0.1 mg/ml
• Purification: Protein G
• Database Link:
  Entrez Gene 8174 Human
• Background: MAdCAM-1 is a cell-surface Ig superfamily member composed of two extracellular Ig domains, followed by a mucin-like domain, a transmembrane domain and a short cytoplasmatic domain. It interacts via its N-terminal Ig domain with the lymphocyte homing receptor alpha4beta7, which plays a critical role in forming the gut-associated lymphoid system. MAdCAM-1 promotes the adhesion of T- and B cells, monocytes/macrophages, and potentially eosinophils, basophils, and differentiated mast cells to the vascular endothelium. Mucosal addressin cell adhesion molecule-1 RNA transcripts are predominantly expressed in the small intestine, mesenteric lymph nodes, colon and spleen; and are very weakly expresssed in human pancreas and brain.
• Synonyms: hMAdCAM-1, MAdCAM-1, Mucosal addressin cell adhesion molecule 1