MADCAM1 Mouse Monoclonal Antibody [Clone ID: 314G8]

CAT#: SM2237PT

MADCAM1 mouse monoclonal antibody, clone 314G8, Purified

Specifications

Product Data

• Clone Name: 314G8
• Applications: ELISA, IHC
• Recommended Dilution: ELISA.
  Immunohistochemistry on Frozen sections: 1/25-1/1000.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Recombinant soluble MAdCAM-1-fc fusion protein.
  Spleen cells from immunised Balb/c mice were fused with cells of the mouse P3X63Ag8.653 myeloma cell line.
• Specificity: This antibody recognises Mucosal Addressin Cell Adhesion Molecule 1 (MAdCAM-1).
  MAdCAM-1 expression has also been reported in the gut lamina propria but Clone 314G8 does not recognise MAdCAM-1 in these tissues.
  Clone 314G8 reacts with the ligand-binding first Ig domain and reports suggest that a splice variant exists in the gut which is not recognised by clone 314G8.
  Clone 314G8 is reported to block the interaction of MAdCAM-1 with alpha 4 beta 7. We recommend the use of SM2237LE for this purpose.
• Formulation: PBS
  State: Purified
  State: Liquid purified IgG fraction from Tissue Culture Supernatant
  Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purification: Affinity Chromatography on Protein G
• Database Link:
  Entrez Gene 8174 Human
• Background: MAdCAM-1 is a 60kD cell surface protein that is involved in lymphocyte trafficking. MAdCAM-1 is expressed on high endothelial venules of Peyers patches and mesenteric lymph nodes. MAdCAM-1 expression has also been reported in the gut lamina propria.
• Synonyms: hMAdCAM-1, MAdCAM-1, Mucosal addressin cell adhesion molecule 1