CD61 / ITGB3 Hamster Monoclonal Antibody [Clone ID: HM beta 3.1]

CAT#: SM2240F

CD61 / ITGB3 hamster monoclonal antibody, clone HM beta 3.1, FITC

Product Images

Staining of mouse peripheral blood platelets with HAMSTER ANTI MOUSE CD61:FITC (SM2240F).

Specifications

Product Data

• Clone Name: HM beta 3.1
• Applications: FC
• Recommended Dilution: Flow Cytometry (Neat-1/10): Use 10 µl of of the suggested working dilution to label 10e6 cells in 100 µl. The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity fc receptors.
• Reactivities: Mouse, Rat
• Host: Hamster
• Isotype: IgG
• Clonality: Monoclonal
• Immunogen: Mouse alpha 5 beta 3 protein purified from the mouse hybridoma 2B4.
  Spleen cells from immunised Armenian hamsters were fused with cells of the P3U1 mouse myeloma cell line.
• Specificity: This antibody recognises the integrin beta 3 subunit (CD61).
  Clone HM beta 3-1 is reported to partially inhibit the binding of CD61 to fibronectin.
  We recommend the use of SM2240LE for this purpose.
• Formulation: PBS, pH 7.4 containing 0.09% Sodium Azide as preservative and 1% BSA as stabilizer.
  Label: FITC
  State: Liquid purified IgG fraction.
  Label: Fluorescein Isothiocyanate Isomer 1
• Concentration: 0.1 mg/ml
• Purification: Affinity Chromatography on Protein G.
• Conjugation: FITC
• Background: CD61 is a 90kD a type I membrane protein, which is expressed primarily on megakaryocytes, platelets, monocytes, macrophages, granulocytes and endothelial cells. CD61 associates with either the alpha IIb integrin (CD41) or the alpha V integrin (CD51) to form the platelet glycoprotein complex IIb/IIIa and the vitronectin receptor (VNR) respectively. The heterodimers formed with CD61 are receptors for a variety of ligands including fibrinogen, fibronectin, von Willebrands factor (vWF), vitronectin and thrombospondin.
• Synonyms: Integrin beta-3, GP3A, GPIIIa