Cd28 Mouse Monoclonal Antibody [Clone ID: E18]

CAT#: SM2266F

Cd28 mouse monoclonal antibody, clone E18, FITC


USD 415.00

5 Days*

Size
    • 100 ug

Product Images

Specifications

Product Data
Clone Name E18
Applications FC
Recommended Dilution Flow Cytometry: neat - 1/5; use 10 µl of the suggested working dilution to label 106 cells in 100 µl.
Reactivities Mouse
Host Mouse
Isotype IgG2b
Clonality Monoclonal
Immunogen Alternate immunisation with A20 cells expressing mCD28 and recombinant mCD28Ig. Spleen cells from immunised CD28-/-knockout mice were fused with cells of the X63Ag8.653 myeloma cell line.
Specificity This antibody recognises CD28, a co-stimulatory cell surface molecule expressed by T cell subsets. Clone E18 has been shown to be able to stimulate murine T lymphocytes in combination with anti-mouse CD3.
Formulation PBS, pH7.4 containing 0.09% Sodium Azide and 1% Bovine Serum Albumin
Label: FITC
State: Liquid purified IgG
Label: Fluorescein Isothiocyanate Isomer 1
Concentration 0.1 mg/ml
Purification Affinity chromatography on Protein G
Conjugation FITC
Gene Name CD28 antigen
Background Human CD28 antigen is a 44 kDa disulfide linked homodimeric T cell specific surface glycoprotein. It is a cell adhesion molecule of the immunoglobulin superfamily which is constitutively expressed on most peripheral blood T lymphocytes (approximately 95% of CD4+ cells and 50% of CD8+ cells). Mature thymocytes exhibit higher levels of CD28 than the immature cells. Activation of T cells results in enhanced CD28 expression. T cell activation requires two combined signals provided by antigen presenting cells. The first is mediated via the T cell receptor following its interaction with antigenic peptide MHC complexes, and the second is delivered by accessory or costimulating molecules through their counter receptors on T lymphocytes. CD28 bears structural homology to CTLA 4 which is expressed at very low levels on the surface of CD4+ and CD8+ peripheral blood cells only following activation. CD28 is the natural receptor for the B7/BB 1 ligand (CD80) a 55-60 kDa glycoprotein which is expressed on activated B lymphocytes, on dendritic cells and on interferon gamma treated monocytes. The binding of B7 1/BB 1 molecules to CD28 is involved in T lymphocyte activation and in the initiation and maintenance of chronic inflammation.
Synonyms TP44
Reference Data

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.