Tissue Factor (F3) Mouse Monoclonal Antibody [Clone ID: CLB/TF-5]

CAT#: SM2300F

Tissue Factor (F3) mouse monoclonal antibody, clone CLB/TF-5, FITC

Specifications

Product Data

• Clone Name: CLB/TF-5
• Applications: FC
• Recommended Dilution: Flow Cytometry: Use 10µl of diluted antibody to label 1x10⁶ cells in 100 µl.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Specificity: This antibody recognises CD142.
  The CLB/TF-5 antibody has been used successfully in an ELISA assay as a coating antibody in association with clone CLB/TF-1 (Cat.-No AM01050PU-N), which recognises a different epitope of CD142.
• Formulation: Tris buffered saline, pH 8.0
  Label: FITC
  State: Liquid purified IgG fraction.
  Stabilizer: 1% BSA
  Preservative: 0.09% Sodium Azide
  Label: Fluorescein Isothiocyanate Isomer I
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein A from tissue culture supernantant.
• Conjugation: FITC
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  This product is photosensitive and should be protected from light.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Database Link:
  Entrez Gene 2152 Human
• Background: CD142 is a 45kD cell surface glycoprotein which is otherwise known as Tissue Factor (TF). CD142 expression can be induced on monocytes, macrophages and endothelial cells by various stimuli including interleukin 1, tumour necrosis factor and endotoxin. CD142 initiates the blood clotting cascade by binding coagulation factor VIIa, which activates factor IX or factor X by specific limited proteolysis. CD142 also plays an important role in inflammation, angiogenesis, and the pathophysiology of atherosclerosis and cancer.
• Synonyms: TF, F3, Coagulation factor III, Thromboplastin