Cd4 (Domain 1) Mouse Monoclonal Antibody [Clone ID: W3/25]

CAT#: SM255F

Cd4 (Domain 1) mouse monoclonal antibody, clone W3/25, FITC

Specifications

Product Data

• Clone Name: W3/25
• Applications: FC
• Recommended Dilution: Flow cytometry: Use 10 µl of neat-1/10 diluted antibody to label 10e6 cells in 100 µl.
  Immunohistochemistry on Frozen and Paraffin Embedded Sections. This clone has been described reacting with paraffin-embedded material following PLP fixation (periodate-lysine-paraformaldehyde) (see Ref. 3).
• Reactivities: Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Rat thymocyte membrane preperation.
• Specificity: This antibody recognises the rat CD4 cell surface glycoprotein, a 55 kD molecule expressed by helper T lymphocytes and weakly by monocytes.
  This antibody inhibits proliferation and IL-2 production in the MLR reaction.
• Formulation: PBS, pH 7.4 with 0.09% Sodium Azide as preservative and 1% BSA as stabilizer.
  Label: FITC
  State: Liquid purified IgG fraction.
  Label: Fluorescein Isothiocyanate Isomer 1
• Concentration: 0.1 mg/ml
• Purification: Affinity Chromatography on Protein G.
• Conjugation: FITC
• Gene Name: Cd4 molecule
• Database Link:
  Entrez Gene 24932 Rat
• Background: CD4 is a single chain transmembraneous glycoprotein (59 kDa) which belongs to the immunoglobulin superfamily. CD4 is present on a subset of T lymphocytes ("helper/inducer" T cells) and is also expressed at a lower level on monocytes, tissue macrophages and granulocytes. The antigen is involved in binding to MHC class II molecules. The intracellular domain of the antigen is associated with p56lck protein tyrosine kinase.
• Synonyms: T-cell surface antigen T4/Leu-3