Cd8a Mouse Monoclonal Antibody [Clone ID: OX-8]

CAT#: SM259R

Cd8a mouse monoclonal antibody, clone OX-8, PE

Specifications

Product Data

• Clone Name: OX-8
• Applications: FC
• Recommended Dilution: Flow Cytometry: Neat - 1/10; Use 10µl of the suggested working dilution to label 10e6 cells in 100µl.
• Reactivities: Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Rat thymocyte membrane glycoproteins. Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.
• Specificity: This antibody recognises the CD8 alpha cell surface antigen, expressed by a subset of T lymphocytes, most thymocytes and the majority of NK cells. Clone MRC OX-8 is suitable for use in in vitro blocking studies. We recommend the use of SM259A for this purpose. This clone has been described reacting with paraffin-embedded material following PLP Fixation (periodatelysine paraformaldehyde)
• Formulation: PBS, pH7.4 containing 0.09% Sodium Azide and 1% Bovine Serum Albumin
  Label: PE
  State: Lyophilized purified IgG
  Label: R. Phycoerythrin (RPE)
• Reconstitution Method: Restore with 1 ml distilled water
• Purification: Affinity chromatography on Protein G
• Conjugation: PE
• Database Link:
  Entrez Gene 24930 Rat
• Background: The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell to cell interactions within the immune system. The CD8 antigen, acting as a coreceptor, and the T cell receptor on the T lymphocyte recognize antigen displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The functional coreceptor is either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains.
• Synonyms: CD8 alpha chain, CD8A, MAL