Pecam1 Mouse Monoclonal Antibody [Clone ID: TLD-3A12]

CAT#: SM272FS

Pecam1 mouse monoclonal antibody, clone TLD-3A12, FITC


USD 260.00

5 Days*

Size
    • 50 ug

Product Images

Specifications

Product Data
Clone Name TLD-3A12
Applications FC
Recommended Dilution Flow Cytometry: Use 10 µl of neat antibody to label 10e6 cells in 100 µl.
Reactivities Rat
Host Mouse
Isotype IgG1
Clonality Monoclonal
Immunogen Activated, Lewis rat derived microglial cells.
Spleen cells from immunised BALB/c mouse were fused with cells of the mouse SP2 myeloma cell line.
Specificity This antibody recognises PECAM-1 (CD31), a type 1 transmembrane protein expressed primarily on endothelial cells, platelets and leucocytes.
Clone TLD-3A12 has been shown to partially block the proliferative response of antigen-specific CD4+ T cells to antigen-presenting cells and relevant antigen (Stevenson, K.S. et al. 2009). We recommend SM272LE for use in functional studies.
This clone has been reported as suitable for use on formalin-fixed paraffin-embedded sections pre-treated with 0.2M boric acid, pH7.0. (Wilson et al. 2007). Mouse anti Rat CD31, clone TLD-3A12 has been shown to be cross-reactive with endothelial cells derived from rhesus macaque (Maclean et al. 2001).
Formulation PBS
Label: FITC
State: Liquid purified IgG fraction from Tissue Culture Supernatant
Stabilizer: 1% BSA
Preservative: 0.09% Sodium Azide
Label: Fluorescein Isothiocyanate Isomer I
Concentration 0.1 mg/ml
Purification Affinity Chromatography on Protein G
Conjugation FITC
Background CD31, also known as platelet endothelial cell adhesion molecule 1 (PECAM1), is a type I integral membrane glycoprotein and a member of the immunoglobulin superfamily of cell surface receptors. It is constitutively expressed on the surface of endothelial cells, and concentrated at the junction between them. It is also weakly expressed on many peripheral lymphoid cells and platelets.
CD31 has been used to measure angiogenesis in association with tumor recurrence. Other studies have also indicated that CD31 and CD34 can be used as markers for myeloid progenitor cells and recognize different subsets of myeloid leukemia infiltrates (granular sarcomas).
Synonyms PECAM-1, EndoCAM, GPIIA'
Reference Data

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.