Ptprc (CD45RC) Mouse Monoclonal Antibody [Clone ID: OX-22]

CAT#: SM280P

Ptprc (CD45RC) mouse monoclonal antibody, clone OX-22, Low Endotoxin

Specifications

Product Data

• Clone Name: OX-22
• Applications: FC, IHC
• Recommended Dilution: Flow Cytometry: Use 10 µl of 1/100 diluted antibody to label 10e6 cells in 100 µl.
  Immunohistochemistry on Frozen Sections:
  Immunohistochemistry on Paraffin Sections:
  This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.
• Reactivities: Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: PHA stimulated rat lymphocytes.
  Spleen cells from immunised BALB/c mice were fused with cells from the NS1 mouse myeloma cell line.
• Specificity: This antibody recognises CD45RC, the high molecular weight form of the leucocyte common antigen.
  The antigen is found on B cells, approximately 50% of bone marrow cells, all CD8+ve T cells, but splits CD4+ve T cells into two populations, CD4+CD45RChigh (Th1-like) and CD4+CD45RClow (Th2-like).
• Formulation: Phosphate Buffered Saline, pH 7.4 without preservatives.
  State: Low Endotoxin
  State: Liquid purified IgG fraction.
• Concentration: 1.0 mg/ml
• Purification: Affinity Chromatography on Protein G.
• Gene Name: Rattus norvegicus protein tyrosine phosphatase, receptor type, C (Ptprc), transcript variant 3
• Database Link:
  Entrez Gene 24699 Rat
• Background: The CD45 family of cell surface glycoprotein antigens exist as a set of isoforms. The patterns of monoclonal antibody recognition of the CD45 phenotypes can be restricted (CD45RA, RB and RC), or broader, such as the CD45 T 200 species. The "restricted" subsets of this group arise as a result of translation of alternatively spliced mRNAs from exons A, B and C (exons 4, 5 and 6), where exons 3-15 encode the extracellular domain of the CD45 molecule. The restricted epitopes are differentially expressed on T and B lymphocytes, including functionally distinct T cells. CD45 (LCA) is a transmembrane phosphotyrosine phosphatase expressed on leucocytes. Myeloid cells do not express the RC isoform.
• Synonyms: PTPRC, Leukocyte common antigen, L-CA, T200