Ptprc (CD45RC) Mouse Monoclonal Antibody [Clone ID: OX-22]

CAT#: SM280PS

Ptprc (CD45RC) mouse monoclonal antibody, clone OX-22, Purified

Specifications

Product Data

• Clone Name: OX-22
• Applications: FC, IHC
• Recommended Dilution: Flow Cytometry: 1/100; Use 10µl of the suggested working dilution to label 10e6 cells in 100µl.
  Immunohistochemistry on frozen sections: 1/100 - 1/1000.
  Immunohistochemistry on paraffins sections: 1/100 - 1/1000; This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.
• Reactivities: Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: PHA stimulated rat lymphocytes. Spleen cells from immunised BALB/c mice were fused with cells from the NS1 mouse myeloma cell line.
• Specificity: This antibody recognises CD45RC, the high molecular weight form of the leucocyte common antigen.
• Formulation: PBS, pH7.4 containing 0.09% Sodium Azide
  State: Purified
  State: Liquid purified IgG
• Concentration: 1.0mg/ml
• Purification: Affinity chromatography on Protein G
• Gene Name: Rattus norvegicus protein tyrosine phosphatase, receptor type, C (Ptprc), transcript variant 3
• Database Link:
  Entrez Gene 24699 Rat
• Background: This antigen is found on B cells, approximately 50% of bone marrow cells, all CD8+ve T cells, but splits CD4+ve T cells into two populations,CD4+ CD45RC (Th 1-like) and CD4+ CD45RC low (Th-2 like).
• Synonyms: PTPRC, Leukocyte common antigen, L-CA, T200