CD71 / TFRC Mouse Monoclonal Antibody [Clone ID: OX-26]

CAT#: SM289B

CD71 / TFRC mouse monoclonal antibody, clone OX-26, Biotin


USD 270.00

2 Weeks*

Size
    • 100 ug

Product Images

Specifications

Product Data
Clone Name OX-26
Applications FC, IHC
Recommended Dilution Flow cytometry.
Immunohistochemistry on frozen sections.
Immunoprecipitation.
Reactivities Rat
Host Mouse
Isotype IgG2a
Clonality Monoclonal
Immunogen PHA activated lymph node cells of PVG rat.
Donor: BALB/c spleen.
Fusion Partner: NS1/1.Ag.4.1.
Specificity This antibody recognizes the transferrin receptor. This 95kDa molecule is found on proliferating cells and brain endothelium.
Formulation PBS, 0.02% NaN3 and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/ml
Label: Biotin
State: Liquid purified Ig
Concentration 0.1 mg/ml
Purification Protein G Chromatography
Conjugation Biotin
Background The transferrin receptor has been structurally characterized as a sulfide bound dimer of identical glycoprotein subunits of 95 kDa. The transferrin receptor is not present on resting blood lymphocytes. On PBL, the receptor appears after activation. The expression of transferrin receptor is coordinately regulated with cell growth. Present on T and B cell lines. The soluble (or serum) transferrin receptor (sTfR) is a circulating truncated form of the membrane receptor protein; it is an 85 kDa glycoprotein forming in serum a 320 kDa complex with diferric transferrin. The most important clinical use of the sTfR determination is in the differential diagnosis between iron deficiency anaemia and the anaemia of chronic disease.
Synonyms TfR1, p90, Transferrin receptor protein 1
Note Protocol: FLOW CYTOMETRY ANALYSIS:

Method:
1. Prepare a cell suspension in media A. For cell preparations, deplete the red blood cell population with Lympholyte®-Rat cell separation medium
2. Wash 2 times.
3. Resuspend the cells to a concentration of 2x10e7 cells/ml in media A. Add 50 µl of this suspension to each tube (each tube will then contain 1 x 10e6 cells, representing 1 test).
4. To each tube, add 1.0 µg* of this Ab per 10e6 cells.
5. Vortex the tubes to ensure thorough mixing of antibody and cells.
6. Incubate the tubes for 30 minutes at 4°C.
7. Wash 2 times at 4°C.
8. Add 100 µl of secondary antibody (Streptavidin-FITC) at a 1:500 dilution.
9. Incubate tubes at 4°C for 30 - 60 minutes (It is recommended that tubes are protected from light since most fluorochromes are light sensitive).
10. Wash 2 times at 4°C.
11. Resuspend the cell pellet in 50 µl ice cold media B.
12. Transfer to suitable tubes for flow cytometric analysis containing 15 µl of propidium iodide at 0.5 mg/ml in PBS. This stains dead cells by intercalating in DNA.

Media:
A. Phosphate buffered saline (pH 7.2) + 5% normal serum of host species + sodium azide (100 µl of 2M sodium azide in 100 mls).
B. Phosphate buffered saline (pH 7.2) + 0.5% Bovine serum albumin + sodium azide (100 µl of 2M sodium azide in 100 mls).

Results - Tissue Distribution:
Rat Strain: Wistar
Cell Concentration: 1x10e6 cells per test
Antibody Concentration Used:1.0 µg/10e6 cells
Isotypic Control: Biotin Mouse IgG2a

Cell Source Percentage of cells stained above control:
Bone Marrow: 14.4%
T Cell Blasts: 86.2%
Spleen: 11.6%
Reference Data

{0} Product Review(s)

0 Product Review(s) Submit review

Be the first one to submit a review

Product Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.