CD161 (KLRB1) Mouse Monoclonal Antibody [Clone ID: 10/78]

CAT#: SM293A

CD161 (KLRB1) mouse monoclonal antibody, clone 10/78, Azide Free

Specifications

Product Data

• Clone Name: 10/78
• Applications: FC, FN, IHC, IP, R, WB
• Recommended Dilution: Western Blot.
  Immunoprecipitation.
  Radioimmunoassays.
  Functional Assays: Clone 10/78 stimulates NK mediated cytoxicity in a manner identical to Clone 3.2.3.
  Flow Cytometry: Use 10 µl of 1/50-1/100 diluted antibody to label 10⁶ in 100 µl.
  Immunohistochemistry on Frozen Sections.
• Reactivities: Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Purified splenic NK cells from the LEW Rat strain.
  Spleen cells from immunised BALB/c mice were fused with cells of the mouse X63.Ag8653 myeloma cell line.
• Specificity: This antibody recognises the CD161 protein (also known as NKR-PI) a 60kD glycoprotein expressed on rat NK cells and T cell subpopulations.
  This monoclonal antibody competes with clone 3.2.3 for binding to the antigen.
• Formulation: PBS, pH7.4
  State: Azide Free
  State: Liquid purified IgG fraction from Tissue Culture Supernatant
  Stabilizer: None
  Preservative: None
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein G
• Storage: Upon receipt, store undiluted (in aliquots) at -20°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Background: Natural killer (NK) cells are lymphocytes that mediate cytotoxicity and secrete cytokines after immune stimulation. Several genes of the C-type lectin superfamily, including the rodent NKRP1 family of glycoproteins, are expressed by NK cells and may be involved in the regulation of NK cell function. The KLRB1 (CD161) protein contains an extracellular domain with several motifs characteristic of C type lectins, a transmembrane domain, and a cytoplasmic domain. The KLRB1 protein is classified as a type II membrane protein because it has an external C terminus.
  In mouse the NKRP1 family has three members, NKRP1A, B and C, whilst in human only one member has been identified. The human protein has received the designation CD161, and the mouse proteins have been referred to as CD161a, b and c. Engagement of CD161c has been reported to have activating function in NK cells, whilst engagement of CD161b is inhibitory.
• Synonyms: HNKR-P1a, CLEC5B, NKRP1A