Bsg Mouse Monoclonal Antibody [Clone ID: OX-47]

CAT#: SM297R

Bsg mouse monoclonal antibody, clone OX-47, PE

Specifications

Product Data

• Clone Name: OX-47
• Applications: FC
• Recommended Dilution: Flow Cytometry: Use 10 µl of Neat antibody to label 10e6 cells in 100 µl.
• Reactivities: Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: T blasts from a mixed lymphocyte reaction.
  Spleen cells from immunised BALB/c mice were fused with cells from the NS0/1 mouse myeloma cell line.
• Specificity: This antibody recognises a membrane glycoprotein of MW 42-48 kD.
  Clone OX-47 is reported to be suitable for use in Western blotting. (4)
• Formulation: PBS, pH 7.4 containing 0.09% Sodium Azide as preservative and 1% BSA as stabilizer.
  Label: PE
  State: Lyophilized purified IgG fraction.
  Label: R. Phycoerythrin (RPE)
• Reconstitution Method: Restore with 1.0 ml distilled water.
• Concentration: 0.1 mg/ml
• Purification: Affinity Chromatography on Protein G.
• Conjugation: PE
• Database Link:
  Entrez Gene 25246 Rat
• Background: OX-47 is expressed at high levels on activated lymphocytes but lower levels on resting cells. The antigen is also present on some endothelia. The antigen contains two Ig-like domains and an unusual transmembrane domain with a charged residue. It is abundant on activated lymphocytes: it is the homologue of the chicken blood brain barrier antigen HT7.(2)
• Synonyms: Basigin, BSG, EMMPRIN, TCSF