Tubulin (TUBA1B) Mouse Monoclonal Antibody [Clone ID: TU-16]

CAT#: SM3001P

Tubulin (TUBA1B) mouse monoclonal antibody, clone TU-16, Purified

Product Images

Immunoprecipitation of alpha-tubulin from HeLa and RAJI cell lysate by antibody TU-16 and its detection by antibody TU-01. IgM heavy chain (76-92 kDa) and IgM light chain (25-30 kDa) indicated. Mr of alpha tubulin is around 50 kDa. L = lysate IPr = immunoprecipitate (reducing conditions)

Specifications

Product Data

• Clone Name: TU-16
• Applications: ELISA, IF, IHC, IP, WB
• Recommended Dilution: Immunoprecipitation.
  Western Blotting (Reducing conditions): 1-2 ug/ml, 60 min.
  Positive Control: HPB-ALL human peripheral blood leukemia cell line; sample preparation: Resuspend approx. 50 mil. cells in 1 ml cold Lysis buffer (1% laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail); Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate with reducing Laemmli SDS-PAGE sample buffer.
  Immunocytochemistry.
  ELISA.
  Immunohistochemistry on Paraffin and Frozen Sections.
  Recommended dilution: 10 ug/ml
• Reactivities: All Species
• Host: Mouse
• Isotype: IgM
• Clonality: Monoclonal
• Immunogen: Porcine brain microtubule protein MTP-1
• Specificity: The antibody reacts with alpha-tubulin alltested species, under denaturing and non-denaturing conditions. (Recognized epitope is conserved within all species).
• Formulation: Phosphate buffered saline (PBS), pH~7.4 with 15 mM Sodium Azide as preservative.
  State: Purified
  State: Liquid purified Ig fraction (> 95% pure by SDS-PAGE).
• Concentration: 1.0 mg/ml
• Purification: Precipitation Methods.
• Gene Name: Homo sapiens tubulin alpha 1b (TUBA1B)
• Database Link:
  Entrez Gene 10376 Human
• Background: The microtubules are intracellular dynamic polymers made up of evolutionarily conserved polymorphic alpha/beta-tubulin heterodimers and a large number of microtubule-associated proteins (MAPs). The microtubules consist of 13 protofilaments and have an outer diameter 25 nm. Microtubules have their intrinsic polarity; highly dynamic plus ends and less dynamic minus ends. Microtubules are required for vital processes in eukaryotic cells including mitosis, meiosis, maintenance of cell shape and intracellular transport. Microtubules are also necessary for movement of cells by means of flagella and cilia. In mammalian tissue culture cells microtubules have their minus ends anchored in microtubule organizing centers (MTOCs).The GTP (guanosintriphosphate) molecule is an essential for tubulin heterodimer to associate with other heterodimers to form microtubule. In vivo, microtubule dynamics vary considerably. Microtubule polymerization is reversible and a populations of microtubules in cells are on their minus ends either growing or shortening this phenomenon is called dynamic instability of microtubules. On a practical level, microtubules can easily be stabilized by the addition of non-hydrolysable analogues of GTP (eg. GMPPCP) or more commonly by anti-cancer drugs such as Taxol. Taxol stabilizes microtubules at room temperature for many hours. Using limited proteolysis by enzymes both tubulin subunits can be divided into N-terminal and C-terminal structural domains. The alpha-tubulin (relative molecular weight about 50 kDa) is globular protein that exists in cells as part of soluble alpha/beta-tubulin dimer or it is polymerized into microtubules. In different species it is coded by multiple tubulin genes that form tubulin classes (in human 6 genes). Expressed tubulin genes are named tubulin isotypes. Some of the tubulin isotypes are expressed ubiquitously, while some have more restricted tissue expression. Alpha-tubulin is also subject of numerous post-translational modifications. Tubulin isotypes and their posttranslational modifications are responsible for multiple tubulin charge variants - tubulin isoforms. Heterogeneity of alpha-tubulin is concentrated in C-terminal structural domain.
• Synonyms: Tubulin alpha-1B chain, Tubulin alpha-ubiquitous chain, Alpha-tubulin ubiquitous, Tubulin K-alpha-1

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Gap junction, Pathogenic Escherichia coli infection