lacZ (Center) Mouse Monoclonal Antibody [Clone ID: BG-02]

CAT#: SM3002P

lacZ (Center) mouse monoclonal antibody, clone BG-02, Purified

Product Images

Western blot of purifiedE. colibeta-galactosidase sample; reduced sample, immunostained bymAbBG-02andgoat anti-mouse IgG (H+L)-HRPconjugate.

Specifications

Product Data

• Clone Name: BG-02
• Applications: IF, WB
• Recommended Dilution: Western blot.
  Immunocytochemistry.
• Reactivities: Escherichia coli
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Beta-Galactosidase purified from E. coli
• Specificity: The antibody recognizes a non-catalytic centre epitope of beta-Galactosidase from E. coli.
• Formulation: PBS, pH~7.4
  State: Purified
  State: Liquid purified Ig fraction from Ascites (> 95% pure by SDS-PAGE)
  Preservative: 15 mM Sodium Azide
• Concentration: 1.0 mg/ml
• Purification: Precipitation methods
• Background: Beta galactosidase is coded by a gene (lac z) in the lac operon of Escherichia coli. It is a metalloenzyme that splits lactose into glucose and galactose. It hydrolyzes terminal, non-reducing beta-D-galactose residues in beta-D-galactosides.
  Activation by cations seems to be substrate dependent. K+, Na+, NH4+, Rb+, Cs+ and Mn++ all activate enzyme activity based upon the substrate used.
• Synonyms: Beta-Gal tag, lacZ tag, b0344, JW0335, Beta-Gal Fusion Protein, Lactase