Bromodeoxyuridine / BrDU Mouse Monoclonal Antibody [Clone ID: MoBu-1]

CAT#: SM3005P

Bromodeoxyuridine / BrDU mouse monoclonal antibody, clone MoBu-1, Purified

Product Images

Immunohistochemistry staining of bromodeoxyuridine-labeled cells (chick embryo; paraffin-embedded sections) with anti-5-bromodeoxyuridine (MoBu-1).

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  Flow cytometry analysis of 5-bromodeoxyuridin (BrdU) incorporation in CEM human acute lymphoblastic leukemia cell line using purified anti-5-bromodeoxyuridin (MoBu-1) (detection by Goat anti-mouse IgG1 FITC). The individual cell cycle phases (S-, G1-, G2/M-phase) are indicated in the figure.

Specifications

Product Data

• Clone Name: MoBu-1
• Applications: FC, IF, IHC
• Recommended Dilution: Flow Cytometry: 1-2 µg/ml.
  Immunocytochemistry: 2 µg/ml.
  Immunohistochemistry on Frozen and Paraffin Sections.
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: 5-bromodeoxyuridine conjugated with hemocyanine
• Specificity: The antibody reacts specifically with BrdU incorporated into DNA during S-phase of a cell cycle.
  The antibody MoBu-1 is also useful for detecting proliferating cells by Flow Cytometry or Immunofluorescence staining. It reacts also specifically with 5-Bromouridine (BrU).
• Formulation: PBS, pH 7.4, with 15 mM Sodium Azide as preservative
  State: Purified
  State: Liquid purified Ig fraction (> 95% pure by PAGE)
• Concentration: 1.0 mg/ml
• Purification: Protein A Affinity Chromatography
• Background: Bromodexyuridine (BrdU) is a thymidine analog which is selectively incorporated into the DNA of proliferating cells to provide a marker for the DNA being replicated. The number of proliferating cells can then be detected in cell lysates, tissue sections or suspensions using an antibody specific for the BrdU.