CD11b (ITGAM) Mouse Monoclonal Antibody [Clone ID: MEM-174]

CAT#: SM3007B

CD11b (ITGAM) mouse monoclonal antibody, clone MEM-174, Biotin

Product Images

Surface staining of human peripheral blood with anti-human CD11b (MEM-174) biotin, streptavidin-APC.

Specifications

Product Data

• Clone Name: MEM-174
• Applications: FC
• Recommended Dilution: Flow cytometry: starting point for indirect immunoflourescence is 1:400.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Human granulocytes
• Specificity: The antibody recognizes CD11b antigen (Mac-1), a 165 kDa type Itransmembrane protein mainly expressed on monocytes, granulocytes and NK-cells. The CD11b mediates neutrophil and monocyte interactions with stimulated endothelium.
• Formulation: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
  Label: Biotin
  State: Liquid purified Ig fraction
  Label: Conjugated with -LC-NHS under optimum conditions. The reagent is free of unconjugated biotin
• Concentration: lot specific
• Conjugation: Biotin
• Storage: Store the antibody undiluted at 2 - 8 °C. DO NOT FREEZE!
• Stability: Shelf life: one year from despatch.
• Gene Name: integrin subunit alpha M
• Database Link:
  Entrez Gene 3684 Human
• Background: CD11b (integrin aM subunit) is a 165 kDa type I transmembrane glycoprotein that non-covalently associates with integrin b2 subunit (CD18); expression of the CD11b chain on the cell surface requires the presence of the CD18 antigen. CD11b/CD18 integrin (Mac-1, CR3) is highly expressed on NK cells, neutrophils, monocytes and less on macrophages. CD11b/CD18 integrin is implicated in various adhesive interactions of monocytes, macrophages and granulocytes, facilitating their diapedesis, as well as it mediates the uptake of complement coated particles, serving as a receptor for the iC3b fragment of the third complement component.
• Synonyms: ITGAM, CR3A, CR-3 alpha chain, Integrin alpha-M, MAC1