CD15 Mouse Monoclonal Antibody [Clone ID: MEM-158]

CAT#: SM3010B

CD15 mouse monoclonal antibody, clone MEM-158, Biotin

Specifications

Product Data

• Clone Name: MEM-158
• Applications: FC
• Recommended Dilution: Suitable for Flow cytometry (1/1000).
• Reactivities: Human
• Host: Mouse
• Isotype: IgM
• Clonality: Monoclonal
• Immunogen: Human granulocytes.
• Specificity: CD15 antigen (Lewisx blood group antigen).
  HLDA Workshop VI: WS Code A053
• Formulation: PBS pH 7.4 with 15 mM sodium azide as preservative.
  Label: Biotin
  State: Liquid
• Concentration: 1 mg/ml
• Purification: Purified
• Conjugation: Biotin
• Background: CD15 (Lewis X, Le(x); stage specific embryonic antigen-1, SSEA-1) is a trisacharide (3-determinant3-fucosyl-N-acetyllactosamine) expressed on several glycolipids, glycoproteins and proteoglycans of various cell types, e.g. granulocytes, mast cells, monocytes, macrophages, cells of gastric mucosa, nervous system or various tumour cells. There are several variants of Lewis x, such as sialyl-Lewis x or sulphated Lewis x. Cells with high surface expression of Le(x) antigen exhibit strong self-aggregation, based on calcium-dependent Le(x)-Le(x) interaction. This process is involved for example in embryo compaction or in autoaggregation of teratocarcinoma cells. Sialyl-Le(x) and its isomer sialyl-Le(a) are ligands of selectins. CD15 expression has been extensively used to confirm diagnosis of Hodgkin´s disease.
• Synonyms: Lewis X, X-Hapten, Lacto-N-Fucopentaose III, Stage-Specific Embryonic Antigen, SSEA1