CD15 Mouse Monoclonal Antibody [Clone ID: MEM-158]

CAT#: SM3010F

CD15 mouse monoclonal antibody, clone MEM-158, FITC

Product Images

Surface staining (flow cytometry) of human peripheral blood cells with anti-human CD15 (MEM-158) FITC. Cells in the granulocyte gate were used for analysis.

Specifications

Product Data

• Clone Name: MEM-158
• Applications: FC
• Recommended Dilution: Suitable for Flow cytometry ( Use 20 µl to label 10e6 cells or 100 µl whole blood).
• Reactivities: Human
• Host: Mouse
• Isotype: IgM
• Clonality: Monoclonal
• Immunogen: Human granulocytes
• Specificity: The antibody reacts with CD15, a cell membrane molecule 3-fucosyl-N-acetyllactosamine (3-FAL) strongly expressed on granulocytes, monocytes, macrophages, mast cells; it is also present on Langerhans cells and some myeloid precursors cells.
• Formulation: Phosphate buffered saline (PBS) containing 15 mM sodium azide and 0.2% (w/v) high-grade protease free Bovine Serum Albumin (BSA) as a stabilizing agent
  Label: FITC
  State: Liquid purified Ig fraction
  Label: Conjugated with Fluorescein isothiocyanate under optimum conditions. The reagent is free of unconjugated
• Conjugation: FITC
• Background: CD15 (Lewis X, Le(x); stage specific embryonic antigen-1, SSEA-1) is a trisacharide determinant (3-fucosyl-N-acetyllactosamine) expressed on several glycolipids, glycoproteins and proteoglycans of various cell types, e.g. granulocytes, mast cells, monocytes, macrophages, cells of gastric mucosa, nervous system or various tumour cells. There are several variants of Lewis x, such as sialyl-Lewis x or sulphated Lewis x. Cells with high surface expression of Le(x) antigen exhibit strong self-aggregation, based on calcium-dependent Le(x)-Le(x) interaction. This process is involved for example in embryo compaction or in autoaggregation of teratocarcinoma cells. Sialyl-Le(x) and its isomer sialyl-Le(a) are ligands of selectins. CD15 expression has been extensively used to confirm diagnosis of Hodgkin's disease.
• Synonyms: Lewis X, X-Hapten, Lacto-N-Fucopentaose III, Stage-Specific Embryonic Antigen, SSEA1