CD15 Mouse Monoclonal Antibody [Clone ID: MEM-158]

CAT#: SM3010P

CD15 mouse monoclonal antibody, clone MEM-158, Purified

Product Images

Surface staining (mass cytometry) of human peripheral blood cells (after ammonium chloride red blood cell lysis) with anti-human CD15 (MEM-158) 173Yb. Gated on singlets.

Specifications

Product Data

• Clone Name: MEM-158
• Applications: FC, IP
• Recommended Dilution: Flow Cytometry.
  Immunoprecipitation.
• Reactivities: Human
• Host: Mouse
• Isotype: IgM
• Clonality: Monoclonal
• Immunogen: Human granulocytes
• Specificity: The antibody MEM-158 reacts with CD15, a cell membrane molecule 3-fucosyl-N-acetyllactosamine (3-FAL) strongly expressed on granulocytes, monocytes, macrophages, mast cells; it is also present on Langerhans cells and some myeloid precursors cells.
• Formulation: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
  State: Purified
  State: Liquid Ig fraction
• Concentration: 1.0 mg/ml
• Purification: Precipitation methods and ion exchange chromatography; purity: > 95% (by SDS-PAGE)
• Background: CD15 (Lewis X, Le(x); stage specific embryonic antigen-1, SSEA-1) is a trisacharide determinant (3-fucosyl-N-acetyllactosamine) expressed on several glycolipids, glycoproteins and proteoglycans of various cell types, e.g. granulocytes, mast cells, monocytes, macrophages, cells of gastric mucosa, nervous system or various tumour cells. There are several variants of Lewis x, such as sialyl-Lewis x or sulphated Lewis x. Cells with high surface expression of Le(x) antigen exhibit strong self-aggregation, based on calcium-dependent Le(x)-Le(x) interaction. This process is involved for example in embryo compaction or in autoaggregation of teratocarcinoma cells. Sialyl-Le(x) and its isomer sialyl-Le(a) are ligands of selectins. CD15 expression has been extensively used to confirm diagnosis of Hodgkin's disease.
• Synonyms: Lewis X, X-Hapten, Lacto-N-Fucopentaose III, Stage-Specific Embryonic Antigen, SSEA1