CD15 Mouse Monoclonal Antibody [Clone ID: MEM-158]

CAT#: SM3010R

CD15 mouse monoclonal antibody, clone MEM-158, PE


USD 300.00

2 Weeks*

Size
    • 100 Tests

Product Images

Specifications

Product Data
Clone Name MEM-158
Applications FC
Recommended Dilution Suitable for Flow Cytometry analysis of human blood cells using 20 μl reagent / 100 μl of whole blood or 10e6 cells in a suspension.
The content of a vial (2 ml) is sufficient for 100 tests.
Reactivities Human
Host Mouse
Isotype IgM
Clonality Monoclonal
Immunogen Human granulocytes.
Specificity The antibody MEM-158 reacts with CD15, a cell membrane molecule 3-fucosyl-N-acetyllactosamine (3-FAL) strongly expressed on granulocytes, monocytes, macrophages, mast cells; it is also present on Langerhans cells and some myeloid precursors cells.
Negative Species: Porcine.
Formulation PBS containing 15 mM sodium azide as preservative and 0.2% (w/v) high-grade BSA (Protease free) as stabilizer.
Label: PE
State: Liquid purified IgG fraction.
Label: Conjugated with R-Phycoerythrin under optimum conditions
Purification Size-exclusion chromatography.
Conjugation PE
Background CD15 (Lewis X, Le(x); stage specific embryonic antigen-1, SSEA-1) is a trisacharide determinant (3-fucosyl-N-acetyllactosamine) expressed on several glycolipids, glycoproteins and proteoglycans of various cell types, e.g. granulocytes, mast cells, monocytes, macrophages, cells of gastric mucosa, nervous system or various tumour cells. There are several variants of Lewis x, such as sialyl-Lewis x or sulphated Lewis x. Cells with high surface expression of Le(x) antigen exhibit strong self-aggregation, based on calcium-dependent Le(x)-Le(x) interaction. This process is involved for example in embryo compaction or in autoaggregation of teratocarcinoma cells. Sialyl-Le(x) and its isomer sialyl-Le(a) are ligands of selectins. CD15 expression has been extensively used to confirm diagnosis of Hodgkin´s disease.
Synonyms Lewis X, X-Hapten, Lacto-N-Fucopentaose III, Stage-Specific Embryonic Antigen, SSEA1
Reference Data

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.