CD16 (FCGR3A) Mouse Monoclonal Antibody [Clone ID: MEM-154]

CAT#: SM3011B

CD16 (FCGR3A) mouse monoclonal antibody, clone MEM-154, Biotin

Specifications

Product Data

• Clone Name: MEM-154
• Applications: FC
• Recommended Dilution: Flow Cytometry: 1/500 as a starting point for Indirect Immunoflourescence.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human granulocytes
• Specificity: This antibody reacts with the epitope on CD16 antigen that residing in proximity to FG loop (probably BC or C'E loop). CD16 is a low affinity receptor for aggregated IgG (FcgRIII antigen). The antibody reacts with CD16+ granulocytes.
• Formulation: PBS, pH~7.4 with 15 mM Sodium Azide as preservative.
  Label: Biotin
  State: Liquid purified Ig fraction.
  Label: Conjugated with -LC-NHS under optimum conditions. The reagent is free of unconjugated biotin
• Concentration: lot specific
• Conjugation: Biotin
• Storage: Store the antibody undiluted at 2 - 8 °C. DO NOT FREEZE!
• Stability: Shelf life: one year from despatch.
• Database Link:
  Entrez Gene 2214 Human
• Background: CD16 (FcgRIII) is a 50-65 kDa glycoprotein serving as a low affinity IgG receptor. Human FcgRIII is expressed in two forms FcgRIII-A and -B. FcgRIII-A is a transmembrane protein of monocytes, macrophages, NK cells and a subset of T cells. It is associated with FceRI-g subunit and is responsible for antibody-dependent NK cell cytotoxicity. Mast cell FcgRIII-A is associated, moreover, with FceRI-b subunit. Besides IgG, FceRI-A can be triggered also by oligomeric IgE. FcgRIII-B is a GPI-linked monomeric receptor expressed on neutrophils and is involved in their activation and induction of a proadhesive phenotype.
• Synonyms: FCGR3A, CD16A, FCG3, FCGR3, IGFR3, Fc-gamma RIII-alpha, Fc-gamma RIII, Fc-gamma RIIIa, FcRIII, FcRIIIa, FcR-10, IgG Fc receptor III-2