CD16 (FCGR3A) Mouse Monoclonal Antibody [Clone ID: MEM-154]

CAT#: SM3011R

CD16 (FCGR3A) mouse monoclonal antibody, clone MEM-154, PE

Product Images

Surface staining of human peripheral blood cells with anti-human CD16 (MEM-154) PE. The antibody MEM-154 does not react with CD16a present on NK cells in many subjects.

Specifications

Product Data

• Clone Name: MEM-154
• Applications: FC
• Recommended Dilution: Flow Cytometry: Analysis of Human blood cells using 20 µl reagent / 100 µl of whole blood or 10⁶ cells in a suspension.
  The content of a vial (2 ml) is sufficient for 100 tests.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human granulocytes
• Specificity: This antibody reacts with the epitope on CD16 antigen that residing in proximity to FG loop (probably BC or C'E loop). CD16 is a low affinity receptor for aggregated IgG (FcgRIII antigen). The antibody reacts with CD16+ granulocytes.
• Formulation: Phosphate buffered saline (PBS) containing 15 mM Sodium Azide as presrevative and 0.2% (w/v) high-grade Protease free BSA as a stabilizing agent.
  Label: PE
  State: Liquid purified IgG fraction
  Label: Conjugated with R-Phycoerythrin under optimum conditions
• Purification: Size-Exclusion Chromatography.
• Conjugation: PE
• Storage: Store the antibody at 2-8°C.
  DO NOT FREEZE!
  This product is photosensitive and should be protected from light.
• Stability: Shelf life: one year from despatch.
• Database Link:
  Entrez Gene 2214 Human
• Background: CD16 (FcgRIII) is a 50-65 kDa glycoprotein serving as a low affinity IgG receptor. Human FcgRIII is expressed in two forms FcgRIII-A and -B. FcgRIII-A is a transmembrane protein of monocytes, macrophages, NK cells and a subset of T cells. It is associated with FceRI-g subunit and is responsible for antibody-dependent NK cell cytotoxicity. Mast cell FcgRIII-A is associated, moreover, with FceRI-b subunit. Besides IgG, FceRI-A can be triggered also by oligomeric IgE. FcgRIII-B is a GPI-linked monomeric receptor expressed on neutrophils and is involved in their activation and induction of a proadhesive phenotype.
• Synonyms: FCGR3A, CD16A, FCG3, FCGR3, IGFR3, Fc-gamma RIII-alpha, Fc-gamma RIII, Fc-gamma RIIIa, FcRIII, FcRIIIa, FcR-10, IgG Fc receptor III-2