Integrin beta 1 (ITGB1) Mouse Monoclonal Antibody [Clone ID: MEM-101A]

CAT#: SM3016B

Integrin beta 1 (ITGB1) mouse monoclonal antibody, clone MEM-101A, Biotin

Product Images

Surface staining of human peripheral blood with anti-human CD29 (MEM-101A) APC.

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  Western Blotting analysis (non-reducing conditions) of isolated peripheral blood lymphocytes of various species using anti-CD29 (MEM-101A). Lane 1: lysate of human PBL Lane 2: lysate of canine PBL Lane 3: lysate of porcine PBL

Specifications

Product Data

• Clone Name: MEM-101A
• Applications: FC
• Recommended Dilution: Flow Cytometry (Indirect Immunoflourescence): Use 1/1000 dilution as starting point.
• Reactivities: Canine, Human, Porcine
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Raji Burkitt's lymphoma cell line
• Specificity: The antibody clone MEM-101A reacts with CD29 (integrin b1 chain), a 130 kDa single chain type I glycoprotein expressed as a heterodimer (non-covalently associated with the integrin a subunits 1-6). CD29 is broadly expressed on majority of hematopoietic and non-hematopoietic cells (leukocytes, platelets, fibroblasts, endothelial cells, epithelial cells and mast cells).
• Formulation: Phosphate buffered saline (PBS), pH~7.4
  Label: Biotin
  State: Liquid purified Ig fraction
  Preservative: 15 mM Sodium Azide
  Label: Conjugated with Biotin-LC-NHS under optimum conditions. The reagent is free of unconjugated biotin.
• Concentration: lot specific
• Conjugation: Biotin
• Storage: Store the antibody undiluted at 2-8°C.
  DO NOT FREEZE!
• Stability: Shelf life: one year from despatch.
• Gene Name: integrin subunit beta 1
• Database Link:
  Entrez Gene 3688 Human
• Background: CD29 (b1 integrin subunit, GPIIa) forms non-covalently linked heterodimers with at least 6 dibberent a chains (a1-a6, CD49a-f) determining the binding properties of b1 (VLA) integrins. These integrins mediate cell adhesion to collagen, fibronectin, laminin and other extracellular matrix (ECM) components. This interaction hinders cell death, whereas disruption of anchorage to ECM leads to apoptosis. Decreased expression of most b1 integrins correlates with acquiring multidrug resistance of tumour cells during selection in presence of antitumour drug. In platelets, translocation of intracellular pool of b1 integrins to the plasma membrane following thrombin stimulation. These integrins are also up-regulated in leukocytes during emigration and extravascular migration and appear to be critically involved in regulating the immune cell trafficking from blood to tissue, as well as in regulating tissue damage and disease symptoms related to inflammatory bowel disease. Through a b1 integrin-dependent mechanism, fibronectin and type I collagen enhance cytokine secretion of human airway smooth muscle in response to IL-1b.
• Synonyms: Fibronectin receptor subunit beta, Integrin VLA-4 subunit beta, ITGB1, FNRB, MDF2, MSK12