CD3E Mouse Monoclonal Antibody [Clone ID: MEM-57]

CAT#: SM3017RD5

CD3E mouse monoclonal antibody, clone MEM-57, PE-DL594

Product Images

Surface staining of human peripheral blood cells with anti-human CD3 (MEM-57) FITC. Cells in the lymphocyte gate were used for analysis.

Specifications

Product Data

• Clone Name: MEM-57
• Applications: FC
• Recommended Dilution: Flow cytometry: Human blood cells using 4 μl reagent / 100 μl of whole blood or 10⁶ cells in a suspension.
  The content of a vial (0.4 ml) is sufficient for 100 tests.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Human thymocytes and T lymphocytes
• Specificity: The antibody MEM-57 reacts with gamma-epsilon and delta-epsilon dimers of human CD3 complex, a part of a bigger multisubunit T cell receptor complex (CD3/TCR) expressed on peripheral blood T lymphocytes and mature thymocytes.
• Formulation: Phosphate buffered saline (PBS)
  Label: PE-DL594
  State: Liquid purified Ig fraction
  Preservative:
  15 mM sodium azide
  
  Label: Conjugated with tandem dye PE-DyLight™ 594 (PE-DL594) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use.
• Conjugation: PE-DL594
• Storage: Store undiluted at 2-8°C. DO NOT FREEZE! This products is photosensitive and should be protected from light.
• Stability: Shelf life: one year from despatch.
• Database Link:
  Entrez Gene 916 Human
• Background: CD3 complex is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases.
• Synonyms: T3/Leu-4
• Note: DyLight™ is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.