CD34 Class III Mouse Monoclonal Antibody [Clone ID: 4H11]
Specifications
Product Data | |
Clone Name | 4H11 |
Applications | FC |
Recommended Dilution | Flow Cytometry analysis of human blood cells using 20 µl reagent / 100 µl sample. Note: This conjugate is routinely tested on permanent CML cell line MOLM-7. |
Reactivities | Human |
Host | Mouse |
Isotype | IgG1 |
Clonality | Monoclonal |
Immunogen | Permanent human cell line derived from peripheral leucocytes of a patient suffering from chronic myeloid leukaemia |
Specificity | The antibody reacts with Class III epitope on CD34 (Mucosialin), a 110-115 kDa monomeric transmembrane phosphoglycoprotein expressed on hematopoietic progenitors cells and on the most pluripotential stem cells; it is gradually lost on progenitor cells. The antibody completely blocks binding of Class II antibody QBEnd10 and Class III antibodies BIRMA K3 and 8G12 on KG1a cell line. |
Formulation | PBS containing 15 mM Sodium Azide as preservative Label: FITC State: Liquid purified Ig fraction Label: Conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC and adjusted for direct use. |
Conjugation | FITC |
Storage | Store the antibody undiluted at 2-8°C. DO NOT FREEZE! This product is photosensitive and should be protected from light. |
Stability | Shelf life: one year from despatch. |
Database Link | |
Background | CD34 is a highly glycosylated monomeric 111-115 kDa surface protein, which is present on many stem cell populations. It is a well established stem cell marker, though its expression on human hematopoietic stem cells is reversible. CD34 probably serves as a surface receptor that undergoes receptor-mediated endocytosis and regulates adhesion, differentiation and proliferation of hematopoietic stem cells and other progenitors. CD34 expression is likely to represent a specific state of hematopoietic development that may have altered adhering properties with expanding and differentiating capabilities in both in vitro and in vivo conditions. |
Synonyms | Hematopoietic progenitor cell marker |
Reference Data |
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