CD34 Class III Mouse Monoclonal Antibody [Clone ID: 4H11]

CAT#: SM3018P

CD34 Class III mouse monoclonal antibody, clone 4H11, Purified

Product Images

Surface staining of Kg-1a human acute myelogenous leukemia cell line with anti-human CD34 (4H11[APG]) FITC. Total viable cells were used for analysis.

Specifications

Product Data

• Clone Name: 4H11
• Applications: FC, IF, IHC, WB
• Recommended Dilution: Flow Cytometry: 2 µg/ml.
  Western blot (non-reducing conditions): 2 µg/ml.
  Positive control: Kg-1a human leukemia cell lysate.
  Negative control: JURKAT human leukemia T-cell line.
  Sample preparation: Resuspend approx. 50 mil. cells in 1 ml cold Lysis buffer (1% laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate with non-reducing SDS-PAGE sample buffer.
  Immunohistochemistry on Paraffin Sections: 10 μg/ml. Positive tissue: placenta endothelium.
  Immunocytochemistry.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Permanent human cell line derived from peripheral leucocytes of a patient suffering from chronic myeloid leukaemia
• Specificity: The antibody reacts with Class III epitope on CD34, a 110-115 kDa monomeric transmembrane phosphoglycoprotein expressed on hematopoietic progenitors cells and on the most pluripotential stem cells; it is gradually lost on progenitor cells.
  The antibody completely blocks binding of Class II antibody QBEnd10 and Class III antibodies BIRMA K3 and 8G12 on KG1a cell line.
• Formulation: PBS pH 7.4 with 15 mM Sodium Azide as preservative
  State: Purified
  State: Liquid purified Ig fraction (> 95% by SDS-PAGE)
• Concentration: lot specific
• Purification: Precipitation methods
• Storage: Store undiluted at 2-8°C.
  DO NOT FREEZE!
  
• Stability: Shelf life: one year from despatch.
• Database Link:
  Entrez Gene 947 Human
• Background: CD34 is a highly glycosylated monomeric 111-115 kDa surface protein, which is present on many stem cell populations. It is a well established stem cell marker, though its expression on human hematopoietic stem cells is reversible. CD34 probably serves as a surface receptor that undergoes receptor-mediated endocytosis and regulates adhesion, differentiation and proliferation of hematopoietic stem cells and other progenitors. CD34 expression is likely to represent a specific state of hematopoietic development that may have altered adhering properties with expanding and differentiating capabilities in both in vitro and in vivo conditions.
• Synonyms: Hematopoietic progenitor cell marker