CD34 Class III Mouse Monoclonal Antibody [Clone ID: 4H11]

CAT#: SM3018R

CD34 Class III mouse monoclonal antibody, clone 4H11, PE

Product Images

Detection of CD34 in human chronic myeloid leukemia cell line MOLM-7 with anti-human CD34 (4H11[APG]) PE.

Specifications

Product Data

• Clone Name: 4H11
• Applications: FC, IF
• Recommended Dilution: Flow Cytometry analysis of human blood cells using 20 μl reagent / 100 μl of whole blood or 10⁶ cells in a suspension.
  Immunocytochemistry / Immunoflourescence.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Permanent human cell line derived from peripheral leucocytes of a patient suffering from chronic myeloid leukaemia
• Specificity: The antibody reacts with Class III epitope on CD34 antigen. The antibody 4H11[APG] completely blocks binding of Class II antibody QBEnd10 and Class III antibodies BIRMA K3 and 8G12 on KG1a cell line.
• Formulation: Phosphate buffered saline (PBS) containing 15 mM sodium azide
  Label: PE
  State: Liquid Ig fraction
  Label: Conjugated with R-Phycoerythrin under optimum conditions
• Purification: Size-exclusion chromatography
• Conjugation: PE
• Storage: Store the antibody at 2 - 8 °C. DO NOT FREEZE! This product is photosensitive and should be protected from light.
• Stability: Shelf life: one year from despatch.
• Database Link:
  Entrez Gene 947 Human
• Background: CD34 is a highly glycosylated monomeric 111-115 kDa surface protein, which is present on many stem cell populations. It is a well established stem cell marker, though its expression on human hematopoietic stem cells is reversible. CD34 probably serves as a surface receptor that undergoes receptor-mediated endocytosis and regulates adhesion, differentiation and proliferation of hematopoietic stem cells and other progenitors. CD34 expression is likely to represent a specific state of hematopoietic development that may have altered adhering properties with expanding and differentiating capabilities in both in vitro and in vivo conditions.
• Synonyms: Hematopoietic progenitor cell marker