CD4 (N-term) Mouse Monoclonal Antibody [Clone ID: MEM-241]

CAT#: SM3020R

CD4 (N-term) mouse monoclonal antibody, clone MEM-241, PE

Product Images

Surface staining of human peripheral blood cells with anti-human CD4 (MEM-241) PE.

Specifications

Product Data

• Clone Name: MEM-241
• Applications: FC
• Recommended Dilution: Flow Cytometry analysis of human blood cells using 20 µl reagent / 100 µl whole blood or 10⁶ cells in a suspension.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: 2 N-terminal domains of human CD4 fused to human IgG1 Fc
• Specificity: The antibody reacts with CD4 antigen, a transmembrane glycoprotein (59 kDa) of the immunoglobulin supergene family present on subset of T lymphocytes ("helper/inducer" T cells) and also expressed at a lower level on monocytes and granulocytes.
• Formulation: Phosphate buffered saline (PBS) containing 15 mM sodium azide
  Label: PE
  State: Liquid purified Ig fraction
  Label: Conjugated with R-Phycoerythrin under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use.
• Conjugation: PE
• Database Link:
  Entrez Gene 920 Human
• Background: CD4 is a single chain transmembrane glycoprotein and belongs to immunoglobulin supergene family. In extracellular region there are 4 immunoglobulin-like domains (1 Ig-like V-type and 3 Ig-like C2-type). Transmembrane region forms 25 aa, cytoplasmic tail consists of 38 aa. Domains 1,2 and 4 are stabilized by disulfide bonds. The intracellular domain of CD4 is associated with p56Lck, a Src-like protein tyrosine kinase. It was described that CD4 segregates into specific detergent-resistant T-cell membrane microdomains. Extracellular ligands: MHC class II molecules (binds to CDR2-like region in CD4 domain 1); HIV envelope protein gp120 (binds to CDR2-like region in CD4 domain 1); IL-16 (binds to CD4 domain 3), Human seminal plasma glycoprotein gp17 (binds to CD4 domain 1), L-selectin.
  Intracellular ligands: p56Lck.
  CD4 is a co-receptor involved in immune response (co-receptor activity in binding to MHC class II molecules) and HIV infection (human immunodeficiency virus; CD4 is primary receptor for HIV-1 surface glycoprotein gp120). CD4 regulates T-cell activation, T/B-cell adhesion, T-cell diferentiation, T-cell selection and signal transduction. Defects in antigen presentation (MHC class II) cause dysfunction of CD4+ T-cells and their almost complete absence in patients blood, tissue and organs (SCID immunodeficiency).
• Synonyms: T-cell surface antigen T4/Leu-3