CD44 Mouse Monoclonal Antibody [Clone ID: MEM-263]

CAT#: SM3024AC

CD44 mouse monoclonal antibody, clone MEM-263, APC

Product Images

Western Blotting analysis (non-reducing conditions) of isolated peripheral blood lymphocytes of various species using anti-CD44 (MEM-263). Lane 1: lysate of human PBL Lane 2: lysate of canine PBL Lane 3: lysate of porcine PBL

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  Surface staining of human peripheral blood using anti-CD44 (MEM-263) APC conjugate.

Specifications

Product Data

• Clone Name: MEM-263
• Applications: FC, WB
• Recommended Dilution: Flow Cytometry analysis of human blood cells using 10 µl reagent/100 µl of whole blood or 10⁶ cells in a suspension.
• Reactivities: Canine, Human, Porcine
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: COS-7 cells (African Green Monkey)
• Specificity: This antibody reacts with extracellular (N-terminal) domain of standard CD44 (Phagocyte glycoprotein 1), a 80-95 kDa transmembrane glycoprotein (hyaladherin family) present on the most of cells and tissues (leukocytes, endothelial cells, mesenchymal cells, etc.); it is negative on platelets and hepatocytes.
• Formulation: Phosphate buffered saline (PBS) solution containing 15mM sodium azide
  Label: APC
  State: Liquid purified Ig fraction
  Label: Conjugated with cross-linked Allophycocyanin under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use.
• Conjugation: APC
• Storage: Store the antibody at 2-8°C.
  DO NOT FREEZE!
  This product is photosensitive and should be protected from light.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens CD44 molecule (Indian blood group) (CD44), transcript variant 1
• Database Link:
  Entrez Gene 960 Human
• Background: CD44 is a transmembrane glycoprotein expressed on the surface of most cells, which serves as a receptor for hyaluronan. CD44 mediates angiogenesis, cell adhesion, proliferation and migration, it is thus important for lymphocyte activation, recirculation and homing, it can thus serve e.g. as a modulator of macrophage recruitment in response to pathogen. Although CD44 functions are essential for physiological activities of normal cells, elevated CD44 expression correlates with poor prognosis in many carcinomas, facilitating tumour growth and metastasis, antiapoptosis and directional motility of cancer cells.
• Synonyms: LHR, MDU2, MDU3, MIC4, CDw44, Epican, ECMR-III, HUTCH-I, Heparan sulfate proteoglycan, Hermes antigen, Hyaluronate receptor, PGP-1

Reference Data

• Protein Families: Adult stem cells, Cancer stem cells, Druggable Genome, Embryonic stem cells, ES Cell Differentiation/IPS, Stem cell relevant signaling - DSL/Notch pathway, Transmembrane
• Protein Pathways: ECM-receptor interaction, Hematopoietic cell lineage