CD45 (PTPRC) Mouse Monoclonal Antibody [Clone ID: MEM-28]

CAT#: SM3025B

CD45 (PTPRC) mouse monoclonal antibody, clone MEM-28, Biotin

Product Images

Surface staining of human peripheral blood cells with anti-human CD45 (MEM-28) biotin.

Specifications

Product Data

• Clone Name: MEM-28
• Applications: FC
• Recommended Dilution: Immunofluorescence analysis by Flow Cytometry: 1: 500 as starting point.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human thymocytes and T lymphocytes
• Specificity: The antibody reacts with all alternative forms of human CD45 antigen (Leukocyte Common Antigen), a 180-220 kDa single chain type I transmembrane protein expressed at high level on all cells of hematopoietic origin, except erythrocytes and platelets.
• Formulation: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
  Label: Biotin
  State: Liquid Ig fraction
  Label: Conjugated with Biotin-LC-NHS under optimum conditions. The reagent is free of unconjugated biotin.
• Concentration: lot specific
• Conjugation: Biotin
• Storage: Store the antibody undiluted at 2 - 8 °C. DO NOT FREEZE!
• Stability: Shelf life: one year from despatch.
• Database Link:
  Entrez Gene 5788 Human
• Background: CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase ubiquitously expressed in all nucleated hematopoietic cells, comprising approximately 10% of all surface proteins in lymphocytes. CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases.
  CD45 protein exists as multiple isoforms as a result of alternative splicing; these isoforms differ in their extracellular domains, whereas they share identical transmembrane and cytoplasmic domains. These isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. Besides the role in immunoreceptor signaling, CD45 is important in promoting cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis.
• Synonyms: PTPRC, Leukocyte common antigen, L-CA, T200