CD47 Mouse Monoclonal Antibody [Clone ID: MEM-122]

CAT#: SM3026P

CD47 mouse monoclonal antibody, clone MEM-122, Purified

Product Images

Surface staining of lysed and washed porcine peripheral blood with purified anti-CD47 (MEM-122) (detection by anti-mouse IgM FITC). Panel A - porcine PBMC stained with Isotype mouse IgM control Panel B, C, D - porcine PBMC sample stained with anti-CD47 (MEM-122) Cells in the granulocyte gate were used for analysis.

Specifications

Product Data

• Clone Name: MEM-122
• Applications: FC, IHC, WB
• Recommended Dilution: Flow Cytometry: 2 μg/ml.
  Western blot (non-Reducing Conditions).
  Immunohistochemistry on Frozen Sections.
• Reactivities: Human
• Host: Mouse
• Isotype: IgM
• Clonality: Monoclonal
• Immunogen: COS-7 (African green monkey) cells.
• Specificity: The antibody reacts with CD47 (Integrin Associated Protein), a 50-55 kDa membrane adhesion molecule (Thrombospondin Receptor; immunoglobulin supergene family) expressed on leukocytes, platelets and erythrocytes. It is also expressed on epithelial cells, endothelial cells, fibroblasts and many tumor cell lines.
• Formulation: PBS, pH~7.4
  State: Purified
  State: Liquid purified Ig fraction (> 95% pure by SDS-PAGE)
  Preservative: 15 mM Sodium Azide
• Concentration: 1.0 mg/ml
• Purification: CHT-Chromatography and Precipitation Methods
• Database Link:
  Entrez Gene 961 Human
• Background: CD47 is an ubiquitously expressed cell surface transmembrane glycoprotein interacting with several integrins and regulating their functions. Engagement of CD47 by soluble ligands or counter receptors modulates various signaling pathways, such as activation of heterotrimeric G proteins. Binding secreted thrombospondin-1, CD47 counteracts graft vascularization. CD47 acts also as a ligand for CD172a (signal regulatory protein a, SIRPa), an immune inhibitory receptor on macrophages; this interaction prevents phagocytosis of CD47-positive cells. Moreover, CD47-CD172a system affects cell migration, B cell adhesion and T cell activation. CD47 is also involved in modulation of chondrocyte responses to mechanical signals, and promotes neuronal development, being especially abundant in synapse-rich regions of brain and retina.
• Synonyms: MER6, IAP, OA3