CD5 Mouse Monoclonal Antibody [Clone ID: MEM-32]

CAT#: SM3027P

CD5 mouse monoclonal antibody, clone MEM-32, Purified


USD 220.00

2 Weeks*

Size
    • 100 ug

Product Images

Specifications

Product Data
Clone Name MEM-32
Applications ELISA, FC, IF, IHC, IP, WB
Recommended Dilution Immunoprecipitation.
Western blot (non reducing conditions).
Immunocytochemistry.
Flow cytometry: 2 μg/ml.
Immunohistochemistry (paraffin sections): 20 μg/ml.
Positive tissue: spleen.
ELISA: Can be used in the Sandwich ELISA as the capture antibody in pair with the detection antibody clone CRIS1.
Reactivities Human
Host Mouse
Isotype IgG1
Clonality Monoclonal
Immunogen Crude thymus membrane fraction
Specificity The antibody MEM-32 reacts with the cell surface glycoprotein CD5, a 67kDa single-chain transmembrane glycoprotein expressed on mature T-lymphocytes, most of thymocytes and B-lymphocytes subset (B-1a lymphocytes).
Formulation Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
State: Purified
State: Liquid Ig fraction
Concentration 1.0 mg/ml
Purification Protein A affinity chromatography; purity: > 95% (by SDS-PAGE)
Gene Name CD5 molecule
Background CD5 antigen (T1; 67 kDa) is a human cell surface T-lymphocyte single-chain transmembrane glycoprotein. CD5 is expressed on all mature T-lymphocytes, most of thymocytes, subset of B-lymphocytes and on many T-cell leukemias and lymphomas. It is a type I membrane glycoprotein whose extracellular region contains three scavenger receptor cysteine-rich (SRCR) domains.
The CD5 is a signal transducing molecule whose cytoplasmic tail is devoid of any intrinsic catalytic activity. CD5 modulates signaling through the antigen-specific receptor complex (TCR and BCR). CD5 crosslinking induces extracellular Ca++ mobilization, tyrosine phosphorylation of intracellular proteins and DAG production. Preliminary evidence shows protein associations with ZAP-70, p56lck, p59fyn, PC-PLC, etc. CD5 may serve as a dual receptor, giving either stimulatory or inhibitory signals depending both on the cell type and development stage. In thymocytes and B1a cells seems to provide inhibitory signals, in peripheral mature T lymhocytes it acts as a costimulatory signal receptor. CD5 is the phenotypic marker of a B cell subpopulation involved in the production of autoreactive antibodies.
Disease relevance: CD5 is a phenotypic marker for some B cell lymphoproliferative
disorders (B-CLL, Hairy cell leukemia, etc.). The CD5+ popuation is expanded in some
autoimmune disorders (Rheumatoid Arthritis, etc.). Herpes virus infections induce loss of
CD5 expression in the expanded CD8+ human T cells.
Synonyms CD5, LEU1
Reference Data

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.