CD55 / DAF Mouse Monoclonal Antibody [Clone ID: MEM-118]

CAT#: SM3030F

CD55 / DAF mouse monoclonal antibody, clone MEM-118, FITC

Product Images

Surface staining of Human peripheral blood leukocytes by Mouse monoclonal anti-CD55 antibody MEM-118.

Specifications

Product Data

• Clone Name: MEM-118
• Applications: FC
• Recommended Dilution: Flow Cytometry: Analysis of Human blood cells using 20 μl reagent/100 μl of whole blood or 10⁶ cells in a suspension.
• Reactivities: Human, Primate
• Host: Mouse
• Isotype: IgM
• Clonality: Monoclonal
• Immunogen: HPB-ALL human T cell line
• Specificity: The antibody recognizes an epitope in SCR4 domain of CD55 (Decay accelerating factor, DAF), a 60-70 kDa glycosylphosphatidylinositol (GPI)-anchored single chain glycoprotein. CD55 is widely expressed on hematopoietic and on many non-hematopoietic cells; it is weakly present on NK cells.
• Formulation: Tris buffered saline (TBS)
  Label: FITC
  State: Liquid purified Ig fraction
  Preservative: 15 mM sodium azide
  Label: Conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC and adjusted for direct use.
• Conjugation: FITC
• Background: CD55 (decay-accelerating factor, DAF) is a GPI-anchored membrane glycoprotein that protects autologous cells from classical and alternative pathway of complement cascade. Bidirectional interactions between CD55 and CD97 are involved in T cell regulation and CD55 can still regulate complement when bound to CD97. In tumours, besides protection agains complement, CD55 promotes neoangiogenesis, tumorigenesis, invasiveness and evasion of apoptosis.