CD63 Mouse Monoclonal Antibody [Clone ID: MEM-259]
Specifications
Product Data | |
Clone Name | MEM-259 |
Applications | FC, IF, IHC, IP, WB |
Recommended Dilution | Flow Cytometry: 2 µg/ml (Detection of activated Platelets, Neutrophils and Basophils). Immunoprecipitation. Western blot (Non-Reducing Conditions). Immunocytochemistry. Immunohistochemistry on Paraffin Sections: 10 µg/ml. Positive tissue: Spleen. |
Reactivities | Human |
Host | Mouse |
Isotype | IgG1 |
Clonality | Monoclonal |
Immunogen | HPB-ALL T cell line |
Specificity | The antibody reacts with CD63 (LAMP-3), a 40-60 kDa tetraspan glycoprotein expressed by granulocytes, platelets, T cells, monocytes/macrophages and endothelial cells. Cell surface exposition of CD63 is usually activation-dependent. |
Formulation | PBS, pH~7.4 State: Purified State: Liquid purified Ig fraction from hybridoma culture supernatant (> 95% pure by SDS-PAGE) Preservative: 15 mM Sodium Azide |
Concentration | lot specific |
Purification | Affinity Chromatography on Protein A |
Storage | Store undiluted at 2-8°C. DO NOT FREEZE! |
Stability | Shelf life: one year from despatch. |
Database Link | |
Background | CD63, a glycoprotein of tetraspanin family, is present in late endosomes, lysosomes and secretory vesicles of various cell types. It is also present in the plasma membrane, usually following cell activation. Hence, it has become an widely used basophil activation marker. In mast cells, however, CD63 exposition does not need their activation. CD63 interacts with integrins and affects phagocytosis and cell migration, it is also involved in H/K-ATPase trafficking regulation of ROMK1 channels. CD63 also serves as a T-cell costimulation molecule. Expression of CD63 can be used for predicting the prognosis in earlier stages of carcinomas. |
Synonyms | OMA81H, Granulophysin, Tetraspanin-30, MLA1, TSPAN30, ME491 |
Reference Data |
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