Cytokeratin 7 (KRT7) (+ KRT17) Mouse Monoclonal Antibody [Clone ID: C-46]

CAT#: SM3046P

Cytokeratin 7 (KRT7) (+ KRT17) mouse monoclonal antibody, clone C-46, Purified

Product Images

Detection of Cytokeratin 7+17 in HeLa cell lysate with monoclonal anti-Cytokeratin 7+17 Antibody (Clone C-46).

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  Immunohistochemistry staining of human breast (paraffin-embedded sections) with anti-cytokeratin 7+17 (C-46).

Specifications

Product Data

• Clone Name: C-46
• Applications: IF, IHC, IP, WB
• Recommended Dilution: Immunoprecipitation.
  Immunocytochemistry.
  Western Blot: 1 µg/ml
  Sample Preparation: Lysis 1% lauryl maltoside fo for 1 hour, 5x10 sec. sonication; 5 min. in hot Laemmli buffer (reducing conditions.)
  Positive Control: HeLa Human cell line.
  Immunohistochemistry on Paraffin Sections: 20 µg/ml.
  Pretreatment: 0.1% pepsin, 30 min, 37°C.
  Incubation: 4°C overnight; Chromogen DAB.
  Positive tissue: Pancreas.
• Reactivities: Bovine, Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Cytoskeleton preparation of HeLa human cervix carcinoma cell line.
• Specificity: The antibody C-46 reacts with Cytokeratin peptides 7 and 17 (54 and 46 kDa).
  Negative Species: Mouse, Rat, Rabbit.
• Formulation: PBS, pH~7.4
  State: Purified
  State: Liquid purified Tissue Culture Supernatant (> 95% by SDS-PAGE)
  Preservative: 15 mM Sodium Azide
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein A
• Storage: Store undiluted at 2-8°C.
  DO NOT FREEZE!
  
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens keratin 7 (KRT7)
• Database Link:
  Entrez Gene 3855 Human
• Background: Cytokeratins are a member of intermediate filaments subfamily represented in epithelial tissues. Cytokeratins are a subfamily of intermediate filaments and characterized by remarkable biochemical diversity. Cytokeratins are represented in epithelial tissues by at least 20 different polypeptides, molecular weight between 40 kDa and 68 kDa. The individual cytokeratin polypeptides are designated 1 to 20 and divided into the type I (acidic cytokeratins 9-20) and type II (basic to neutral cytokeratins 1-8) families.
• Synonyms: KRT7, KRT-7, SCL, CK-7, CK7, Keratin-7, K7, K-7, Sarcolectin

Reference Data

• Protein Families: ES Cell Differentiation/IPS