Human Kappa Light Chain Mouse Monoclonal Antibody [Clone ID: MEM-09]

CAT#: SM3064P

Human Kappa Light Chain mouse monoclonal antibody, clone MEM-09, Aff - Purified

Product Images

Western Blotting analysis (reducing conditions) of human blood sera using anti-human Immunoglobulin kappa-chain (MEM-09). Lane 1-3: Human blood serum of different healthy donors

Specifications

Product Data

• Clone Name: MEM-09
• Applications: ELISA, FC, IF, IHC, IP, WB
• Recommended Dilution: Flow Cytometry: 1 µg/ml.
  Immunoprecipitation.
  Western Blot.
  Immunohistochemistry on Paraffin Sections.
  Immunocytochemistry.
  ELISA.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Crude thymus membrane fraction.
• Specificity: The antibody reacts with both secreted and B cell-surface Human immunoglobulin, specifically reacts with kappa-light chains. Material immunoprecipitated from Human serum with the antibody MEM-09 consists of IgG and traces of IgM.
• Formulation: PBS, pH~7.4
  State: Aff - Purified
  State: Liquid Ig fraction (> 95 % pure by SDS-PAGE)
  Preservative: 15mM Sodium Azide
• Concentration: 1.0 mg/ml
• Purification: Affinity Chromatography on Protein A
• Background: Immunoglobulin classes share the same basic four polypeptide chain structure of two heavy chains (five heavy chains types) and two light chains (kappa, lambda; both having a molecular weight of 22.5 kDa). Kappa and lambda consist of a variable region and a constant region and can easily be differentiated by the antigenic properties of the constant region. The ratio of kappa to lambda is 70:30.