SIT (SIT1) Mouse Monoclonal Antibody [Clone ID: SIT-01]

CAT#: SM3074P

SIT (SIT1) mouse monoclonal antibody, clone SIT-01, Purified

Product Images

Western blot of human Jurkat T cell line lysate (1% laurylmaltoside); non-reduced sample, immunostained bymAbSIT-01 andgoat anti-mouse IgG (H+L)-HRPconjugate.

Specifications

Product Data

• Clone Name: SIT-01
• Applications: FC, IP, WB
• Recommended Dilution: Flow Cytometry (intracellular staining).
  Immunoprecipitation.
  Western Blot: Western blot analysis demonstrated that SIT is expressed as an approximately 40 kDa protein that is reduced to approximately 20 kDa by endoglycosidase treatment.
• Reactivities: Human, Mouse
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Bacterially produced recombinant intracellular fragment of human SIT
• Specificity: The antibody reacts with SHP2-interacting transmembrane adaptor protein (SIT) expressed exclusively in lymphoid organs.
• Formulation: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
  State: Purified
  State: Liquid Ig fraction
• Concentration: 1.0 mg/ml
• Purification: Protein-A affinity chromatography; purity: > 95% (by SDS-PAGE)
• Gene Name: Homo sapiens signaling threshold regulating transmembrane adaptor 1 (SIT1)
• Database Link:
  Entrez Gene 27240 Human
• Background: SIT (SHP2-interacting transmembrane adaptor protein) is expressed exclusively in lymphoid organs and acts either as a positive or as a negative regulatory element in T cell activation and in T cell development. Binding to Grb2 plays a pivotal role in signal transduction. Hubener et al. (2001) determined that the SIT gene contains 5 exons and spans 1.8 kb of genomic DNA. The SIT promoter demonstrated strong transcriptional activity and potential binding sites for both ubiquitous and lymphoid-specific transcription factors.
• Synonyms: 40; gp30; MGC125908; MGC125909; MGC125910; RP11-331F9.5; SIT

Reference Data

• Protein Families: Transmembrane