CD52 Mouse Monoclonal Antibody [Clone ID: HI186]

CAT#: SM3081R

CD52 mouse monoclonal antibody, clone HI186, PE

Product Images

Surface staining of CD52 in human peripheral blood with anti-CD52 (HI186) PE.

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  Figure 2. CD52 negative pro T ALL (gate leukemic cells).

Specifications

Product Data

• Clone Name: HI186
• Applications: FC
• Recommended Dilution: Flow Cytometry: Use 20 µl to label 10⁶ cells in a suspension or 100 µl whole blood.
  The content of a vial (2 ml) is sufficient for 100 tests.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Human tonsil.
• Specificity: This antibody reacts with Human CD52 (CAMPATH-1), a 21-28 kDa glycoprotein containing a large N-linked carbohydrate moiety; mature CD52 molecule is actually much smaller (approx. 8-9 kDa). CD52 is expressed at high levels on lymphocytes, Monocytes/Macrophages and in male reproductive tract.
• Formulation: PBS
  Label: PE
  State: Liquid purified IgG fraction
  Stabilizer: 0.2% (w/v) high-grade protease free BSA
  Preservative: 15 mM Sodium Azide
  Label: Conjugated with R-Phycoerythrin under optimum conditions
• Purification: Size-Exclusion Chromatography
• Conjugation: PE
• Storage: Store the antibody at 2-8°C.
  DO NOT FREEZE!
  This product is photosensitive and should be protected from light.
• Stability: Shelf life: one year from despatch.
• Database Link:
  Entrez Gene 1043 Human
• Background: CD52 is a highly glycosylated GPI-anchored 21-28 kDa glycopeptide which is present at high levels on lymphocytes, macrophages, epithelial cells of male reproductive tract and mature sperm. Its 12-amino acid beckbone carries a complex N-linked carbohydrate moiety, which differs between sperm and leukocyte CD52, as well as the GPI anchor does. CD52 can be acquired by sperm cells from seminal plasma, where it is released by epithelial cells. Although CD52 is not an essential T-cell costimulator, its triggering results in activation of normal human T cells. CD52 is a very good target for antibody/complement-mediated cell lysis.
• Synonyms: HE5, CDw52