CD33 Mouse Monoclonal Antibody [Clone ID: HIM3-4]

CAT#: SM3084B

CD33 mouse monoclonal antibody, clone HIM3-4, Biotin

Product Images

Surface staining of human peripheral blood leukocytes with anti-human CD33 (HIM3-4) biotin / streptavidin-APC.

Specifications

Product Data

• Clone Name: HIM3-4
• Applications: FC
• Recommended Dilution: Indirect Immunofluorescence analysis by Flow Cytometry (1/1000).
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: NFMY-9s Human cell line.
• Specificity: The antibody HIM3-4 reacts with CD33, a 67 kDa type I transmembrane glycoprotein (immunoglobulin superfamily) expressed on myeloid progenitors, monocytes, granulocytes, dendritic cells and mast cells; it is absent on platelets, lymphocytes, erythrocytes and hematopoietic stem cells.
  HLDA V; WS Code M MA112.
  HLDA VI; WS Code M MA47.
• Formulation: PBS, pH~7.4
  Label: Biotin
  State: Liquid purified Ig fraction
  Preservative: 15 mM Sodium Azide
  Label: -LC-NHS, The reagent is free of unconjugated Biotin.
• Concentration: lot specific
• Purification: Affinity Chromatography
• Conjugation: Biotin
• Storage: Store undiluted at 2-8°C.
  DO NOT FREEZE!
  
• Stability: Shelf life: one year from despatch.
• Gene Name: CD33 molecule
• Database Link:
  Entrez Gene 945 Human
• Background: CD33 is a transmembrane protein of the sialic acid-binding immunoglobulin-like lectin (Siglec) family. It belongs to the immunoreceptor tyrosine-based inhibitory motif (ITIM)-containing molecules able of recruiting protein tyrosine phosphatases SHP-1 and SHP-2 to signal assemblies; these ITIMs are also used for ubiquitin-mediated removal of the receptor from the cell surface. CD33 is expressed on cells of myelomonocytic lineage, binds sialic acid residues in N- and O-glycans on cell surfaces, and is a therapeutic target for acute myeloid leukemia.
• Synonyms: Siglec-3, gp67