CD11a (ITGAL) Mouse Monoclonal Antibody [Clone ID: MEM-83]

CAT#: SM3100P

CD11a (ITGAL) mouse monoclonal antibody, clone MEM-83, Purified

Specifications

Product Data

• Clone Name: MEM-83
• Applications: FC, FN, IP
• Recommended Dilution: Flow Cytometry: 1 µg/ml.
  Immunoprecipitation.
  Functional Application: The antibody MEM-83 directly induces the binding of T cells to purified ICAM-1. Using an in vitro-translated CDlla cDNA deletion series, the MEM-83 activation epitope was mapped to the "I" domain of the LFA-1 a subunit. The studies have therefore identified a novel LFA-1 activation epitope mapping to the I domain of LFA-1, which could play a role in the regulation of LFA-1 binding to ICAM-1.
  
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human peripheral blood lymphocytes
• Specificity: The antibody recognizes an unique epitope on CD11a antigen (LFA-1 alpha chain) implicated in activation of LFA-1 complex.
• Formulation: Phosphate buffered saline (PBS), pH~7.4
  State: Purified
  State: Liquid purified Ig fraction (> 95% pure by SDS-PAGE)
  Preservative: 15 mM Sodium Azide
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein A
• Storage: Store undiluted at 2-8°C.
  DO NOT FREEZE!
  
• Stability: Shelf life: one year from despatch.
• Gene Name: integrin subunit alpha L
• Database Link:
  Entrez Gene 3683 Human
• Background: CD11a (LFA-1a) together with CD18 constitute leukocyte function-associated antigen 1 (LFA-1), the aLb2 integrin. CD11a is implicated in activation of LFA-1 complex. LFA-1 is
  expressed on the plasma membrane of leukocytes in a low-affinity conformation. Cell stimulation by chemokines or other signals leads to induction the high-affinity conformation, which supports tight binding of LFA-1 to its ligands, the intercellular adhesion molecules ICAM-1, -2, -3. LFA-1 is thus involved in interaction of various immune cells and in their tissue-specific settlement, but participates also in control of cell differentiation and proliferation and of T-cell effector functions. Blocking of LFA-1 function by specific antibodies or small molecules has become an important therapeutic approach in treatment of multiple inflammatory diseases. For example, humanized anti-LFA-1 antibody Efalizumab (Raptiva) is being used to interfere with T cell migration to sites of inflammation; binding of cholesterol-lowering drug simvastatin to CD11a allosteric site leads to immunomodulation and increase in lymphocytic cholinergic activity.
• Synonyms: Integrin alpha-L, LFA1, LFA-1