CD18 (ITGB2) Mouse Monoclonal Antibody [Clone ID: MEM-48]

CAT#: SM3103B

CD18 (ITGB2) mouse monoclonal antibody, clone MEM-48, Biotin

Product Images

Western Blotting analysis (non-reducing conditions) of whole cell lysate using anti-human CD18 (MEM-48). Lane 1: KG-1a human acute myeloid leukemia cell line Lane 2: HPB-ALL human peripheral blood T cell leukemia cell line

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  Immunohistochemistry staining of human spleen (paraffin sections) using anti-CD18 (MEM-48).

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  Surface staining of human peripheral blood cells with anti-human CD18 (MEM-48) PE. Cells in the granulocyte gate were used for analysis.

Specifications

Product Data

• Clone Name: MEM-48
• Applications: FC, FN, IHC, IP, WB
• Recommended Dilution: Flow cytometry.
  Immunoprecipitation.
  Western blot: 2 µg/ml. Positive control: Kg-1a human leukemia cell lysate. Sample preparation: buffer with laurylmaltoside, 2 x non-reducing SDS. Application note: Non-reducing conditions. SDS-PAGE (6% separating gel).
  Immunohistochemistry on frozen sections.
  Functional application: The antibody MEM-48 induces high-affinity conformation of LFA-1 complex.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Leukocytes of a patient suffering from a LGL-type leukemia.
• Specificity: This antibody recognizes an epitope involving residues 534-546 in cysteine-rich repeat 3 of the CD18 antigen (integrin b2 subunit; b2 integrin). CD18 is a 90-95 kDa type I transmembrane protein expressed on all leukocytes.
• Formulation: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
  Label: Biotin
  State: Liquid Ig fraction
• Concentration: lot specific
• Purification: Protein-A affinity chromatography; purity > 95% (by SDS-PAGE)
• Conjugation: Biotin
• Storage: Store the antibody undiluted at 2 - 8 °C. DO NOT FREEZE!
• Stability: Shelf life: one year from despatch.
• Database Link:
  Entrez Gene 3689 Human
• Background: CD18, integrin b2 subunit, forms heterodimers with four types of CD11 molecule to constitute leukocyte (b2) integrins: aLb2 (CD11a/CD18, LFA-1), aMb2 (CD11b/CD18, Mac-1, CR3), aXb2 (CD11c/CD18) and aDb2 (CD11d/CD18). In most cases, the response mediated by the integrin is a composite of the functions of its individual subunits. These integrins are essential for proper leukocyte migration, mediating intercellular contacts. Absence of CD18 leads to leukocyte adhesion deficiency-1; severe reduction of CD18 expression leads to the development of a psoriasiform skin disease. CD18 is also a target of Mannheimia (Pasteurella) haemolytica leukotoxin and is sufficient to mediate leukotoxin-mediated cytolysis.
• Synonyms: Integrin beta-2, MFI7