CD18 (ITGB2) Mouse Monoclonal Antibody [Clone ID: MEM-48]

CAT#: SM3103B

CD18 (ITGB2) mouse monoclonal antibody, clone MEM-48, Biotin


USD 270.00

2 Weeks*

Size
    • 100 ug

Product Images

Specifications

Product Data
Clone Name MEM-48
Applications FC, FN, IHC, IP, WB
Recommended Dilution Flow cytometry.
Immunoprecipitation.
Western blot: 2 µg/ml. Positive control: Kg-1a human leukemia cell lysate. Sample preparation: buffer with laurylmaltoside, 2 x non-reducing SDS. Application note: Non-reducing conditions. SDS-PAGE (6% separating gel).
Immunohistochemistry on frozen sections.
Functional application: The antibody MEM-48 induces high-affinity conformation of LFA-1 complex.
Reactivities Human
Host Mouse
Isotype IgG1
Clonality Monoclonal
Immunogen Leukocytes of a patient suffering from a LGL-type leukemia.
Specificity This antibody recognizes an epitope involving residues 534-546 in cysteine-rich repeat 3 of the CD18 antigen (integrin b2 subunit; b2 integrin). CD18 is a 90-95 kDa type I transmembrane protein expressed on all leukocytes.
Formulation Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Label: Biotin
State: Liquid Ig fraction
Concentration lot specific
Purification Protein-A affinity chromatography; purity > 95% (by SDS-PAGE)
Conjugation Biotin
Storage Store the antibody undiluted at 2 - 8 °C. DO NOT FREEZE!
Stability Shelf life: one year from despatch.
Background CD18, integrin b2 subunit, forms heterodimers with four types of CD11 molecule to constitute leukocyte (b2) integrins: aLb2 (CD11a/CD18, LFA-1), aMb2 (CD11b/CD18, Mac-1, CR3), aXb2 (CD11c/CD18) and aDb2 (CD11d/CD18). In most cases, the response mediated by the integrin is a composite of the functions of its individual subunits. These integrins are essential for proper leukocyte migration, mediating intercellular contacts. Absence of CD18 leads to leukocyte adhesion deficiency-1; severe reduction of CD18 expression leads to the development of a psoriasiform skin disease. CD18 is also a target of Mannheimia (Pasteurella) haemolytica leukotoxin and is sufficient to mediate leukotoxin-mediated cytolysis.
Synonyms Integrin beta-2, MFI7
Reference Data

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.