CD71 (TFRC) Mouse Monoclonal Antibody [Clone ID: MEM-75]

CAT#: SM3104R

CD71 (TFRC) mouse monoclonal antibody, clone MEM-75, PE

Product Images

Surface staining of CD71 in K562 cells (red) and human lymphocytes (negative, grey) using anti-CD71 (MEM-75) PE.

Specifications

Product Data

• Clone Name: MEM-75
• Applications: FC
• Recommended Dilution: Flow Cytometry analysis of human blood cells using 20 μl reagent / 100 μl of whole blood or 10⁶ cells in a suspension.
  The content of a vial (2 ml) is sufficient for 100 tests.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: NALM-6 human pre-B cell line
• Specificity: The antibody MEM-75 reacts with CD71 antigen (transferrin receptor), a 95 kDa type II homodimeric transmembrane glycoprotein expressed on activated B and lymphocytes, macrophages and erythroid precursors; it is lost on resting blood leukocytes.
  The antibody MEM-75 does not block binding of transferrin to the receptor.
• Formulation: PBS containing 15 mM sodium azide as preservative and 0.2% (w/v) high-grade BSA (Protease free) as stabilizer.
  Label: PE
  State: Liquid purified IgG fraction
  Label: Conjugated with R-Phycoerythrin under optimum conditions
• Purification: Size-exclusion chromatography
• Conjugation: PE
• Storage: Store the antibody in the dark at 2-8°C.
  Do Not Freeze!
  Avoid prolonged exposure to light.
• Stability: Shelf life: one year from despatch.
• Gene Name: transferrin receptor
• Database Link:
  Entrez Gene 7037 Human
• Background: CD71 (transferrin receptor) is a type II transmembrane glycoprotein expressed as homodimer in erythroid blood cell line and in activated leukocytes. Upon binding of holotransferrin (complex of transferrin and iron ions), CD71 is internalized by clathrin-mediated endocytosis. Acidification of endosomes by vesicular membrane proton pumps leads to dissociation of iron ions, whereas transferrin (apotransferrin) remains associated with CD71 and recycles to the cell surface, where it is released upon exposure to normal pH. CD71 is also involved in uptake of non-transferrin bound iron.
• Synonyms: TfR1, p90, Transferrin receptor protein 1