HLAE (HLA-E) Mouse Monoclonal Antibody [Clone ID: MEM-E/06]

CAT#: SM3113P

HLAE (HLA-E) mouse monoclonal antibody, clone MEM-E/06, Purified

Product Images

Immunohistochemistry staining of human spleen (paraffin sections) with anti-HLA-E (clone MEM-E/06).

Specifications

Product Data

• Clone Name: MEM-E/06
• Applications: FC, IHC, IP
• Recommended Dilution: Flow Cytometry.
  Immunoprecipitation.
  Immunohistochemistry on paraffin sections: 10 μg/ml; positive tissue: spleen.
• Reactivities: Human, Primate
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Bacterially expressed recombinant HLA-E refolded with beta2-microglobulin and peptide
• Specificity: The antibody recognized native surface-expressed HLA-E, but not denaturated heavy chain of HLA-E. The published results showed that the antibody cross-reacts with some classical MHC Class I molecules (HLA-A3, -A11, -B7). However, the recent Workshop I Session on the 3rd International Conference on HLA-G (Paris, July 2003) confirmed that the antibody exhibits much broader cross-reactivity classical MHC Class I antigens, namely with HLA-A24, -A32, -B8, -B15, -B27, -B35, -B44, -B54, -C3, -C4, -C5, -C7.
• Formulation: Tris buffered saline (TBS) with 15 mM sodium azide, approx. pH 8.0
  State: Purified
  State: Liquid Ig fraction
• Concentration: lot specific
• Purification: Protein-A affinity chromatography; purity: > 95% (by SDS-PAGE)
• Storage: Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Database Link:
  Entrez Gene 3133 Human
• Background: HLA-E belongs to the MHC Class I molecules (MHC Class Ib; nonclassical) and it is expressed on many types of the human cells.
• Synonyms: HLA-6.2, HLAE, MHC class I antigen E