NTAL (LAT2) Mouse Monoclonal Antibody [Clone ID: NAP-07]

CAT#: SM3122P

NTAL (LAT2) mouse monoclonal antibody, clone NAP-07, Purified

Product Images

Subcellular localization of NTAL by confocal microscopy in THP-1 human acute monocytic leukemia cell line. THP-1 cells were permeabilized and immunostained using anti-NTAL (NAP-07; green). Nuclei are vizualized by propidium iodide (red).

Specifications

Product Data

• Clone Name: NAP-07
• Applications: FC, IF, IP, WB
• Recommended Dilution: Flow Cytometry:
  Positive tissue: RAJI Human lymphoma cell line. RAMOS Human lymphoma cell line
  Immunoprecipitation.
  Western Blot: 2 µg/ml, incubation 60 min.
  Positive Control: RAMOS Human lymphoma cell line.
  Negative Control: HeLa Human cervix carcinoma cell line.
  Sample preparation: Resuspend approx. 50 mil. cells in 1 ml cold Lysis buffer (1% laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate with non-reducing SDS-PAGE sample buffer.
  Application note: Non-Reducing conditions. 12% separating SDS-PAGE gel.
  Immunocytochemistry.
• Reactivities: Human, Mouse
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Recombinant cytoplasmic domain (aa 91-244) of human NTAL
• Specificity: The antibody reacts with Non-T cell activation linker (NTAL), also known as LAB (linker of activated B cells), a 25 - 30 kDa transmembrane adaptor protein present in membrane microdomains (rafts) of B lymphocytes, NK cells and myeloid cells.
• Formulation: Phosphate buffered saline (PBS) with 15 mM Sodium Azide, pH~7.4
  State: Purified
  State: Liquid purified Ig fraction (> 95% pure by SDS-PAGE)
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein A
• Storage: Store undiluted at 2-8°C.
  DO NOT FREEZE!
  
• Stability: Shelf life: one year from despatch.
• Database Link:
  Entrez Gene 7462 Human
• Background: NTAL (Non-T cell activation linker), also known as LAB (Linker for activation of B cells), is a 30 kDa double-palmitoylated transmembrane adaptor protein expressed by B cells, NK cells, mast cells and macrophages. It is a negative regulator of early stages of BCR-dependent B cell signaling and serves as a negative regulator also in mast cells.
  However, in mast cells, NTAL also contributes to some activation processes, partially overlapping with LAT function.
• Synonyms: LAB, NTAL, WBS15, WBSCR5, HSPC046