Glycophorin A (GYPA) (N-term) Mouse Monoclonal Antibody [Clone ID: HIR2]

CAT#: SM3141F

Glycophorin A (GYPA) (N-term) mouse monoclonal antibody, clone HIR2, FITC

Product Images

Surface staining of human peripheral blood with anti-CD235a (HIR2) PE.

Specifications

Product Data

• Clone Name: HIR2
• Applications: FC
• Recommended Dilution: Flow Cytometry analysis of Human blood cells using 20 µl reagent / 100 µl of whole blood or 10⁶ cells in a suspension.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Synthetic peptide (Human, N-terminal)
• Specificity: This antibody recognizes N-terminal portion of glycophorin A (and weakly of glycophorin B). Its antigen is expressed on early erythroblasts, late erythroblasts, erythroblasts, mature erythrocytes and the cells of erythroid cell lines K562 and HEL, but not on all other cells.
• Formulation: Phosphate buffered saline (PBS) solution containing 15mM sodium azide
  Label: FITC
  State: Liquid purified Ig fraction
  Label: Conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC and adjusted for direct use
• Conjugation: FITC
• Storage: Store undiluted at 2-8°C.
  DO NOT FREEZE!
  This product is photosensitive and should be protected from light.
• Stability: Shelf life: one year from despatch.
• Gene Name: glycophorin A (MNS blood group)
• Database Link:
  Entrez Gene 2993 Human
• Background: CD235a (Glycophorin A, GPA) is a transmembrane sialoglycoprotein expressed on erythrocytes and their precursors. Similarly to glycophorin B (GPB), these molecules provide the cells with a large mucin-like surface, which minimalizes aggregation between erythrocytes in the circulation. GPA is the carrier of blood group M and N specificities, while GPB accounts for S, s and U specificities. CD235a is a receptor of Hsa, an Streptococcus adhesin.
• Synonyms: Glycophorin-A, GPA, PAS-2, Sialoglycoprotein alpha, MN sialoglycoprotein